LOCUS       sequence-285543-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3000
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(881..905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             913..954
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gtcagcagcg gccggcgagg
       61 aagttaccca gtctcctcct ggacccgacg gaggagacgg ttcgccgtcg gtgccgagac
      121 cccatcaacg tggagggcct gctgccatca aaaataagga ttaatttaga agataatgta
      181 caatatgtgt ccatgagaaa agctctaaaa gtgaagagac ctcgttttga tgtatcgctg
      241 gtttatttaa ctcgaaaatt tatggatctt gtcagatctg ctcccggggg tattcttgac
      301 ttaaacaagg ttgcaacgaa actgggagtc cgaaagcgga gagtgtatga catcaccaat
      361 gtcttagatg gaatcgacct cgttgaaaag aaatccaaga accatattag atggatagga
      421 tctgatctta gcaattttgg agcagttccc caacaaaaga agctacagga ggaactttct
      481 gacttatcag caatggaaga tgctttggat gagttaatta aggattgtgc tcagcagctg
      541 tttgagttaa cagatgacaa agaaaatgaa agactagcat atgtgaccta tcaagacatt
      601 catagcattc aggccttcca tgaacagatc gtcattgcag ttaaagctcc agcagaaacc
      661 agattggatg ttccagctcc cagagaagac tctatcacag tgcacataag gagcaccaac
      721 ggacctatcg atgtctattt gtgtgaagtg gagcagggtc agaccagtaa caaaaggtct
      781 gaaggtgtcg ggacctcttc atctgagagc actcatccag aaggccctga ggaagaagaa
      841 aatcctcagc aaagtgaaga attgcttgaa gtaagcaact gcccaacttt cttgtacaaa
      901 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      961 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1021 gacgagagaa gaatgctgta caaccttga
//
