LOCUS       sequence-285546-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3001
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(566..590)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             598..639
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cttcctcgtc tggcaacgat
       61 gatgatctca ctatccccag agctgctatc aataaaatga tcaaagagac tcttcctaat
      121 gtccgggtgg ccaacgatgc tcgagagctg gtggtgaact gctgcactga attcattcac
      181 cttatatctt ctgaagccaa tgagatttgt aacaaatcgg aaaagaagac catctcacca
      241 gagcatgtca tacaagcact agaaagtttg ggatttggct cttacatcag tgaagtaaaa
      301 gaagtcttgc aagagtgtaa aacagtagca ttaaaaagaa gaaaggccag ttctcgtttg
      361 gaaaaccttg gcattcctga agaagagtta ttgagacagc aacaagaatt atttgcaaaa
      421 gctagacagc aacaagcaga attggcccaa caggaatggc ttcaaatgca gcaagctgcc
      481 caacaagccc agcttgctgc tgcctcagcc agtgcatcta atcaggcggg atcttctcag
      541 gatgaagaag atgatgatga tatctgccca actttcttgt acaaagtggt tgatatcggt
      601 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      661 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtgatttata
      721 taacactggt aaac
//
