LOCUS       sequence-285549-        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3002
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1043..1067)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1075..1116
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ccacagaagg aggatttggt
       61 ggtactagca gcagtgatgc ccagcaaagc ctacagtcgt tctggcctcg ggtcatggaa
      121 gaaatccgga atttaacagt gaaagacttc cgagtgcagg aactcccact ggctcgtatt
      181 aagaagatta tgaaactgga tgaagatgtg aagatgatca gtgcagaagc gcctgtactc
      241 tttgccaagg cagcccagat ttttatcaca gagttgactc ttcgagcctg gattcacaca
      301 gaagataaca agcgccggac tctacagaga aatgatatcg ccatggcaat tacaaaattt
      361 gatcagtttg attttctcat cgatattgtt ccaagagatg aactgaaacc tccaaagcgt
      421 caggaggagg tgcgccagtc tgtaactcct gccgagccag tccagtacta tttcacgctg
      481 gctcagcaac ccaccgctgt ccaagtccag ggccagcagc aaggccagca gaccaccagc
      541 tccacgacca ccatccagcc tgggcagatc atcatcgcac agcctcagca gggccagacc
      601 acacctgtga caatgcaggt tggagaaggt cagcaggtgc agattgtcca ggctcagcca
      661 cagggtcaag cccaacaggc ccagagtggc actggacaga ccatgcaggt gatgcagcag
      721 atcatcacta acacaggaga gatccagcag atcccggtgc agctgaatgc cggccagctg
      781 cagtatatcc gcttagccca gcctgtatca ggcactcaag ttgtgcaggg acagatccag
      841 acacttgcca ccaatgctca acagattaca cagacagagg tccagcaagg acagcagcag
      901 ttcagccagt tcacagatgg acagcagctc taccagatcc agcaagtcac catgcctgcg
      961 ggccaggacc tcgcccagcc catgttcatc cagtcagcca accagccctc cgacgggcag
     1021 gccccccagg tgaccggcga ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1081 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1141 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac attttgatga
     1201 ttcggttacg c
//
