LOCUS       sequence-285552-        1319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3003
FEATURES             Location/Qualifiers
     source          1..1319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1151..1175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1183..1224
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccaattca tgaacttctc
       61 agcctttatg gttatggtag tactgttcga ctacctgaag aagatgagga agaggaagaa
      121 gaggaagaag aaggtgaaga tgatgaagat gctgataatg atgacaacag tggctgtagt
      181 ggggaaaata aagaggagaa tataaaggat tcatcaggtc aggaggatga aactcagtct
      241 tccaatgatg atccatcaca atctgttgct tctcaagatg cccaggaaat aatccgccca
      301 cgtcgatgta aatattttga tacaaatagt gaagtagaag aagaatctga agaagatgaa
      361 gattatattc catcagaaga ctggaaaaag gagattatgg tgggctccat gtttcaagca
      421 gaaattccag ttggcatttg tagatacaaa gaaaatgaaa aagtatatga aaatgatgat
      481 cagctcctgt gggaccctga gtacttacca gaagataaag tgattatatt tcttaaagat
      541 gcatctagaa gaacaggtga tgagaagggt gtagaagcaa ttcctgaagg atctcacata
      601 aaagacaatg aacaggcttt atatgaattg gttaaatgca attttgatac agaagaagca
      661 ttgagaagat taagatttaa tgtaaaagca gctagagagg aattatctgt ttggacagag
      721 gaagagtgta gaaattttga acaagggctg aaggcctatg gaaaggattt tcatttgatt
      781 caggctaata aagtccgaac aaggtcagtt ggtgaatgtg tagcattcta ttacatgtgg
      841 aaaaaatctg aacgttatga tttctttgct cagcaaacac gatttggaaa gaagaaatat
      901 aatcttcatc ctggtgtaac ggattacatg gatcgtcttc tagacgaaag tgaaagtgct
      961 gcatctagtc gagcaccatc ccctccccca actgcatcaa acagtagtaa cagccagtct
     1021 gagaaagaag atggcactgt aagcactgct aatcaaaatg gagtgtcatc taatggacca
     1081 ggcatactcc aaatgcttct tccagttcat ttttcagcca tcagttcaag agccaatgcc
     1141 tttttaaaat tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1201 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1261 atttcttggc tttatatatc ttgtggaaag gacgactttt gccgttttac cgcaaagca
//
