LOCUS       sequence-285555-        1238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3004
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1070..1094)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1102..1143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc atcggactac acggatcaaa
       61 atcacagagc tgaaccccca cctcatgtgt gccctctgcg gggggtactt catcgacgcc
      121 accactatcg tggagtgcct gcattccttc tgcaaaacct gcatcgtgcg ctacctggag
      181 accaacaaat actgccccat gtgtgacgtg caggtccata aaacccggcc gctgctgagc
      241 atcaggtctg acaaaacact tcaagacatt gtctacaaat tggtccctgg gctttttaaa
      301 gatgagatga aacggcggcg ggatttctat gcagcgtacc ccctgacgga ggtccccaac
      361 ggctccaatg aggaccgcgg cgaggtcttg gagcaggaga agggggctct gagtgatgat
      421 gagattgtca gcctctccat cgaattctac gaaggtgcca gggaccggga cgagaagaag
      481 ggccccctgg agaatgggga tggggacaaa gagaaaacag gggtgcgctt cctgcgatgc
      541 ccagcagcca tgaccgtcat gcatcttgcc aagtttctcc gcaacaagat ggatgtgccc
      601 agcaagtaca aggtggaggt tctgtacgag gacgagccac tgaaggaata ctacaccctc
      661 atggacatcg cctacatcta cccctggcgg cggaacgggc ctctccccct caagtaccgt
      721 gtccagccag cctgcaagcg gctcacccta gccacggtgc ccaccccctc cgagggcacc
      781 aacaccagcg gggcgtccga gtgtgagtca gtcagcgaca aggctcccag ccctgccacc
      841 ctgccagcca cctcctcctc cctgcccagc ccagccaccc catcccatgg ctctcccagt
      901 tcccatgggc ctccagccac ccaccctacc tcccccactc ccccttcgac agccagtggg
      961 gccaccacag ctgccaacgg gggtagcttg aactgcctgc agacaccatc ctccaccagc
     1021 agggggcgca agatgactgt caacggcgct cccgtgcccc ccttaacttg cccaactttc
     1081 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1141 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1201 tgtggaaagg acgaggacat tataacaatc tagaacct
//
