LOCUS       sequence-285558-        1862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3005
FEATURES             Location/Qualifiers
     source          1..1862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1694..1718)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1726..1767
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acaatatgtc tattacgaat
       61 acaccaacaa gtaatgatgc ctgtctgagc attgtgcata gtttgatgtg ccatagacaa
      121 ggtggagaga gtgaaacatt tgcaaaaaga gcaattgaaa gtttggtaaa gaagctgaag
      181 gagaaaaaag atgaattgga ttctttaata acagctataa ctacaaatgg agctcatcct
      241 agtaaatgtg ttaccataca gagaacattg gatgggaggc ttcaggtggc tggtcggaaa
      301 ggatttcctc atgtgatcta tgcccgtctc tggaggtggc ctgatcttca caaaaatgaa
      361 ctaaaacatg ttaaatattg tcagtatgcg tttgacttaa aatgtgatag tgtctgtgtg
      421 aatccatatc actacgaacg agttgtatca cctggaattg atctctcagg attaacactg
      481 cagagtaatg ctccatcaag tatgatggtg aaggatgaat atgtgcatga ctttgaggga
      541 cagccatcgt tgtccactga aggacattca attcaaacca tccagcatcc accaagtaat
      601 cgtgcatcga cagagacata cagcacccca gctctgttag ccccatctga gtctaatgct
      661 accagcactg ccaactttcc caacattcct gtggcttcca caagtcagcc tgccagtata
      721 ctggggggca gccatagtga aggactgttg cagatagcat cagggcctca gccaggacag
      781 cagcagaatg gatttactgg tcagccagct acttaccatc ataacagcac taccacctgg
      841 actggaagta ggactgcacc atacacacct aatttgcctc accaccaaaa cggccatctt
      901 cagcaccacc cgcctatgcc gccccatccc ggacattact ggcctgttca caatgagctt
      961 gcattccagc ctcccatttc caatcatcct gctcctgagt attggtgttc cattgcttac
     1021 tttgaaatgg atgttcaggt aggagagaca tttaaggttc cttcaagctg ccctattgtt
     1081 actgttgatg gatacgtgga cccttctgga ggagatcgct tttgtttggg tcaactctcc
     1141 aatgtccaca ggacagaagc cattgagaga gcaaggttgc acataggcaa aggtgtgcag
     1201 ttggaatgta aaggtgaagg tgatgtttgg gtcaggtgcc ttagtgacca cgcggtcttt
     1261 gtacagagtt actacttaga cagagaagct gggcgtgcac ctggagatgc tgttcataag
     1321 atctacccaa gtgcatatat aaaggtcttt gatttgcgtc agtgtcatcg acagatgcag
     1381 cagcaggcgg ctactgcaca agctgcagca gctgcccagg cagcagccgt ggcaggaaac
     1441 atccctggcc caggatcagt aggtggaata gctccagcta tcagtctgtc agctgctgct
     1501 ggaattggtg ttgatgacct tcgtcgctta tgcatactca ggatgagttt tgtgaaaggc
     1561 tggggaccgg attacccaag acagagcatc aaagaaacac cttgctggat tgaaattcac
     1621 ttacaccggg ccctccagct cctagacgaa gtacttcata ccatgccgat tgcagaccca
     1681 caacctttag actgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1741 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1801 tcgatttctt ggctttatat atcttgtgga aaggacgatc gtcacacagc ccggtgttgg
     1861 ga
//
