LOCUS       sequence-285561-        1583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3006
FEATURES             Location/Qualifiers
     source          1..1583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(580..591)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1415..1439)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1447..1488
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cctctggggt ggaagtcctg
       61 cgcttccagc tgcccggcca cgaggccgca acgctacgga acatgaacca gctccgggca
      121 gaggagcggt tctgcgacgt gaccattgtg gccgacagcc tcaagtttcg aggccacaag
      181 gtcatcttgg ccgcctgctc acccttcctg cgggaccagt tcctgctgaa ccccagctcg
      241 gagctgcagg tctccctgat gcacagtgca cgcatcgtgg ccgacttgct cctctcctgc
      301 tacacgggcg ccttggaatt cgctgttagg gacatcgtca actaccttac agccgcctcc
      361 tacctgcaga tggagcacgt ggtggagaaa tgccggaatg ccctcagcca gttcattgag
      421 cccaaaatag gcctcaaaga ggatggggtc agtgaggcta gccttgtgag cagcatcagc
      481 gccaccaagt ccctcctccc tccagccagg accccaaagc cagccccgaa gcccccaccc
      541 ccacctcctc taccccctcc actcctgcgg ccagtgaagc tggagttccc actggatgaa
      601 gacttggagc tgaaagccga ggaagaggat gaggatgagg atgaggacgt gtctgacatc
      661 tgcatcgtca aggtggagtc ggccctggag gtggcacacc ggctcaaacc ccctggaggc
      721 ctgggagggg gtctgggcat tggaggctcc gtgggtggcc accttgggga gctggcccag
      781 agcagcgttc cccccagcac tgtagcccca ccgcagggtg tggtgaaggc ctgctatagc
      841 ctgtcggaag atgcagaagg ggagggcctg ctgttgattc ccggaggccg ggccagcgtg
      901 ggggccacct cgggcctggt ggaagcagca gcggtggcca tggctgcccg gggggcgggg
      961 ggcagcctgg gggcgggggg cagccgggga cccctgcctg ggggcttctc aggtggaaac
     1021 cccttaaaga acatcaagtg caccaagtgc ccggaagtgt tccagggcgt ggagaagctg
     1081 gtcttccaca tgcgggcgca gcacttcatc ttcatgtgcc ctcgctgtgg caagcagttc
     1141 aaccacagca gcaacctcaa ccgccacatg aacgtgcatc gtggtgtcaa gtcacactcg
     1201 tgcggcatct gcggcaagtg cttcacacag aagtccaccc ttcacgacca cctcaacctg
     1261 cactcgggag cgcggcccta ccgctgctcc tactgcgacg tgcgcttcgc ccacaagcct
     1321 gccattaggc ggcacctcaa ggagcaacac ggcaagacca cggccgagaa cgtgctggag
     1381 gccagtgtgg ccgagattaa cgtcctcatc cgctacccaa ctttcttgta caaagtggtt
     1441 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1501 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1561 tgaccgtaaa atggcttgcc tgt
//
