LOCUS       sequence-285564-        1601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3007
FEATURES             Location/Qualifiers
     source          1..1601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1433..1457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1465..1506
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga atgtgacaag tttattttcc
       61 tttacaagtc cagctgtgaa gagacttctt gggtggaaac agggcgatga agaagaaaaa
      121 tgggcagaga aagctgttga tgctttggtg aaaaaactga agaaaaagaa aggtgccatg
      181 gaggaactgg aaaaggcctt gagctgccca gggcaaccga gtaactgtgt caccattccc
      241 cgctctctgg atggcaggct gcaagtctcc caccggaagg gactgcctca tgtcatttac
      301 tgccgtgtgt ggcgctggcc cgatcttcag agccaccatg aactaaaacc actggaatgc
      361 tgtgagtttc cttttggttc caagcagaag gaggtctgca tcaatcccta ccactataag
      421 agagtagaaa gccctgtact tcctcctgtg ctggttccaa gacacagcga atataatcct
      481 cagcacagcc tcttagctca gttccgtaac ttaggacaaa atgagcctca catgccactc
      541 aacgccactt ttccagattc tttccagcaa cccaacagcc acccgtttcc tcactctccc
      601 aatagcagtt acccaaactc tcctgggagc agcagcagca cctaccctca ctctcccacc
      661 agctcagacc caggaagccc tttccagatg ccagctgata cgcccccacc tgcttacctg
      721 cctcctgaag accccatgac ccaggatggc tctcagccga tggacacaaa catgatggcg
      781 cctcccctgc cctcagaaat caacagagga gatgttcagg cggttgctta tgaggaacca
      841 aaacactggt gctctattgt ctactatgag ctcaacaatc gtgtgggtga agcgttccat
      901 gcctcctcca caagtgtgtt ggtggatggt ttcactgatc cttccaacaa taagaaccgt
      961 ttctgccttg ggctgctctc caatgttaac cggaattcca ctattgaaaa caccaggcgg
     1021 catattggaa aaggagttca tctttattat gttggagggg aggtgtatgc cgaatgcctt
     1081 agtgacagta gcatctttgt gcaaagtcgg aactgcaact accatcatgg atttcatcct
     1141 actactgttt gcaagatccc tagtgggtgt agtctgaaaa tttttaacaa ccaagaattt
     1201 gctcagttat tggcacagtc tgtgaaccat ggatttgaga cagtctatga gcttacaaaa
     1261 atgtgtacta tacgtatgag ctttgtgaag ggctggggag cagaatacca ccgccaggat
     1321 gttactagca ccccctgctg gattgagata catctgcacg gccccctcca gtggctggat
     1381 aaagttctta ctcaaatggg ttcacctcat aatcctattt catctgtatc ttacccaact
     1441 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1501 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1561 tcttgtggaa aggacgaagt tcctacgcta atacatgcct t
//
