LOCUS       sequence-285570-        1790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3009
FEATURES             Location/Qualifiers
     source          1..1790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1622..1646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1654..1695
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgactgcaga attgagagaa
       61 gccatggccc tagccccatg gggcccagtg aaggtgaaaa aggaggagga agaagaagaa
      121 aacttcccag gtcaggcatc cagccaacaa gtgcactccg agaacatcaa agtctgggcc
      181 ccagtgcagg gtcttcagac aggccttgat ggatcagaag aggaagaaaa gggtcagaac
      241 atatcctggg atatggcggt agtcctgaaa gcaactcagg aggcacctgc tgcttcaacc
      301 cttggcagct actcattacc agggactctg gccaagagtg agatactgga gactcatggg
      361 accatgaact ttctaggtgc tgaaaccaag aacctacagt tactggttcc aaaaactgag
      421 atatgtgagg aagctgaaaa acccctcatc atatcagaaa gaatccagaa agctgatcct
      481 caaggacctg agttaggaga agcttgtgaa aagggaaaca tgttaaagag gcagagaata
      541 aagagagaaa agaaagattt cagacaagtg atagtgaatg actgtcactt acctgaaagc
      601 ttcaaagaag aggaaaacca gaaatgtaag aaatctggag gaaaatatag ccttaattct
      661 ggcgctgtta aaaatccaaa aacccagctt ggacaaaagc cttttacgtg tagcgtgtgt
      721 gggaaaggat ttagtcagag tgcaaacctc gttgtgcatc agcgaatcca cactggagag
      781 aaaccctttg aatgtcatga gtgtgggaag gccttcattc agagtgcaaa cctcgttgtg
      841 catcagagaa tccacactgg acagaaacct tatgtttgct caaaatgtgg gaaagccttc
      901 actcagagtt caaatctgac tgtacatcaa aaaatccact ccttagaaaa aacttttaag
      961 tgcaatgaat gtgagaaagc ctttagttac agctcacaac ttgctcggca ccagaaagtc
     1021 cacattacgg aaaaatgcta tgaatgtaat gaatgtggga aaacatttac taggagctca
     1081 aacctcattg tccaccagag gatccacact ggggagaagc cctttgcctg taacgactgt
     1141 ggcaaagcct ttacccagag tgcaaatctt attgtacatc agcgaagcca tactggtgag
     1201 aagccatatg agtgtaaaga gtgtgggaaa gcctttagtt gtttttcaca ccttattgtg
     1261 caccagagaa ttcacactgc agagaaacct tacgactgca gcgaatgtgg gaaagccttc
     1321 agtcagctct cttgccttat tgtccaccag agaattcaca gtggagatct tccttacgtg
     1381 tgtaatgaat gtgggaaggc cttcacatgt agctcatacc tacttattca tcagagaatt
     1441 cataatggag aaaaacctta cacatgtaat gagtgtggga aggccttcag acagaggtcg
     1501 agcctcaccg tgcaccagag aacccacact ggggagaagc cctatgaatg tgagaagtgt
     1561 ggtgcagctt tcatttccaa ctcacacctc atgcgacacc atagaaccca tcttgttgaa
     1621 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1681 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1741 ctttatatat cttgtggaaa ggacgataca ttttataaat gttagcgccc
//
