LOCUS       sequence-285573-        1262 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3010
FEATURES             Location/Qualifiers
     source          1..1262
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1094..1118)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1126..1167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccagtaggga agatgagctg
       61 aggaactgtg tggtatgtgg ggaccaagcc acaggctacc actttaatgc gctgacttgt
      121 gagggctgca agggtttctt caggagaaca gtcagcaaaa gcattggtcc cacctgcccc
      181 tttgctggaa gctgtgaagt cagcaagact cagaggcgcc actgcccagc ctgcaggttg
      241 cagaagtgct tagatgctgg catgaggaaa gacatgatac tgtcggcaga agccctggca
      301 ttgcggcgag caaagcaggc ccagcggcgg gcacagcaaa cacctgtgca actgagtaag
      361 gagcaagaag agctgatccg gacactcctg ggggcccaca cccgccacat gggcaccatg
      421 tttgaacagt ttgtgcagtt taggcctcca gctcatctgt tcatccatca ccagcccttg
      481 cccaccctgg cccctgtgct gcctctggtc acacacttcg cagacatcaa cactttcatg
      541 gtactgcaag tcatcaagtt tactaaggac ctgcctgtct tccgttccct gcccattgaa
      601 gaccagatct cccttctcaa gggagcagct gtggaaatct gtcacatcgt actcaatacc
      661 actttctgtc tccaaacaca aaacttcctc tgcgggcctc ttcgctacac aattgaagat
      721 ggagcccgtg tatctcccac agtggggttc caggtagagt ttttggagtt gctctttcac
      781 ttccatggaa cactacgaaa actgcagctc caagagcctg agtatgtgct cttggctgcc
      841 atggccctct tctctcctga ccgacctgga gttacccaga gagatgagat tgatcagctg
      901 caagaggaga tggcactgac tctgcaaagc tacatcaagg gccagcagcg aaggccccgg
      961 gatcggtttc tgtatgcgaa gttgctaggc ctgctggctg agctccggag cattaatgag
     1021 gcctacgggt accaaatcca gcacatccag ggcctgtctg ccatgatgcc gctgctccag
     1081 gagatctgca gctgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1141 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1201 tcgatttctt ggctttatat atcttgtgga aaggacgaac gtcttccaat ccctctgctc
     1261 ta
//
