LOCUS       sequence-285576-        1925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3011
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..261
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     protein_bind    complement(1757..1781)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1789..1830
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atgctaagtc actaactgcc
       61 tggtcccgga cactggtgac cttcaaggat gtatttgtgg acttcaccag ggaggagtgg
      121 aagctgctgg acactgctca gcagatcgtg tacagaaatg tgatgctgga gaactataag
      181 aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg gttggagaag
      241 ggagaagagc cctggctggt ggagagagaa attcaccaag agacccatcc tgattcagag
      301 actgcatttg aaatcaaatc atcagtttcc agcaggagca tttttaaaga taagcaatcc
      361 tgtgacatta aaatggaagg aatggcaagg aatgatctct ggtatttgtc attagaagaa
      421 gtctggaaat gtagagacca gttagacaag tatcaggaaa acccagagag acatttgagg
      481 caagtggcat tcacccaaaa gaaagtactt actcaggaga gagtctctga aagtggtaaa
      541 tatgggggaa actgtcttct tcctgctcag ctagtactga gagagtattt ccataaacgt
      601 gactcacata ctaaaagttt aaaacatgat ttagttctta atggtcatca ggacagttgt
      661 gcaagtaaca gtaatgaatg tggtcaaact ttctgtcaaa acattcacct tattcagttt
      721 gcaagaactc acacaggtga taaatcctac aaatgccctg ataatgacaa ctctcttact
      781 catggttcat ctcttggtat atcaaagggc atacatagag agaaacccta tgaatgtaag
      841 gaatgtggaa aattcttcag ctggcgctct aatcttacta ggcatcagct tattcatact
      901 ggagaaaaac cgtatgagtg taaagaatgt ggaaagtctt tcagccggag ttctcacctc
      961 attggacatc aaaagaccca tactggtgag gaaccctatg aatgtaaaga atgtggaaaa
     1021 tccttcagct ggttctctca ccttgttact catcagagaa ctcatacagg agacaaactg
     1081 tacacatgta atcagtgtgg gaaatctttt gttcatagct ctaggcttat tagacaccag
     1141 aggacacata ctggagagaa accctatgaa tgtcctgaat gtgggaaatc tttcagacag
     1201 agcacacatc tcattctgca tcagagaacc catgtgagag tgaggcccta tgaatgcaat
     1261 gaatgtggaa agtcttacag ccagagatct caccttgttg tgcatcatag aattcacact
     1321 ggactaaaac cttttgagtg taaggattgt ggaaaatgtt ttagtcgaag ctctcacctt
     1381 tattcacatc aaagaaccca cactggagag aaaccatatg agtgtcatga ttgtggaaaa
     1441 tctttcagcc agagttctgc ccttattgtg catcagagga tacacactgg agagaaacca
     1501 tatgaatgct gtcagtgtgg gaaagccttc atccggaaga atgacctcat taagcaccag
     1561 agaattcatg ttggagaaga gacctataaa tgtaatcaat gtggcattat cttcagccag
     1621 aactctccat ttatagttca tcaaatagct cacactggag agcagttctt aacatgcaat
     1681 caatgtggga cagcgcttgt taatacctct aaccttattg gataccagac aaatcatatt
     1741 agagaaaatg cttactaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1801 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1861 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgtttcatt ctgtgccacc
     1921 cggca
//
