LOCUS       sequence-285579-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3012
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(728..752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             760..801
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agcagagttc gaacgtgccg
       61 gctttcctca gcaagctgtg gacgcttgtg gaggaaaccc acactaacga gttcatcacc
      121 tggagccaga atggccaaag ttttctggtc ttggatgagc aacgatttgc aaaagaaatt
      181 cttcccaaat atttcaagca caataatatg gcaagctttg tgaggcaact gaatatgtat
      241 ggtttccgta aagtagtaca tatcgactct ggaattgtaa agcaagaaag agatggtcct
      301 gtagaatttc agcatcctta cttcaaacaa ggacaggatg acttgttgga gaacattaaa
      361 aggaaggttt catcttcaaa accagaagaa aataaaattc gtcaggaaga tttaacaaaa
      421 attataagta gtgctcagaa ggttcagata aaacaggaaa ctattgagtc caggctttct
      481 gaattaaaaa gtgagaatga gtccctttgg aaggaggtgt cagaattacg agcaaagcat
      541 gcacaacagc aacaagttat tcgaaagatt gtccagttta ttgttacatt ggttcaaaat
      601 aaccaacttg tgagtttaaa acgtaaaagg cctctacttc taaacactaa tggagcccaa
      661 aagaagaacc tgtttcagca catagtcaaa gaaccaactg ataatcatca tcataaagta
      721 attttttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      781 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      841 tcttggcttt atatatcttg tggaaaggac gagatcaaga acgccaggat tcagcc
//
