LOCUS       sequence-285582-        1703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3013
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1535..1559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1567..1608
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ggcggaagaa aatacaaatc
       61 acacgcataa tggatgaaag gaaccgacag gtcactttta caaagagaaa gtttggatta
      121 atgaagaaag cctatgaact tagtgtgctc tgtgactgtg aaatagcact catcattttc
      181 aacagctcta acaaactgtt tcaatatgct agcactgata tggacaaagt tcttctcaag
      241 tatacagaat ataatgaacc tcatgaaagc agaaccaact cggatattgt tgaggctctg
      301 aacaagaagg aacacagagg gtgcgacagc ccagaccctg atacttcata tgtgctaact
      361 ccacatacag aagaaaaata taaaaaaatt aatgaggaat ttgataatat gatgcggaat
      421 cataaaatcg cacctggtct gccacctcag aacttttcaa tgtctgtcac agttccagtg
      481 accagcccca atgctttgtc ctacactaac ccagggagtt cactggtgtc cccatctttg
      541 gcagccagct caacgttaac agattcaagc atgctctctc cacctcaaac cacattacat
      601 agaaatgtgt ctcctggagc tcctcagaga ccaccaagta ctggcaatgc aggtgggatg
      661 ttgagcacta cagacctcac agtgccaaat ggagctggaa gcagtccagt ggggaatgga
      721 tttgtaaact caagagcttc tccaaatttg attggagcta ctggtgcaaa tagcttaggc
      781 aaagtcatgc ctacaaagtc tccccctcca ccaggtggtg gtaatcttgg aatgaacagt
      841 aggaaaccag atcttcgagt tgtcatcccc ccttcaagca agggcatgat gcctccacta
      901 aatacccaaa ggatcagtag ttctcaagcc actcaacctc ttgctacccc agtcgtgtct
      961 gtgacaaccc caagcttgcc tccgcaagga cttgtgtact cagcaatgcc gactgcctac
     1021 aacactgatt attcactgac cagcgctgac ctgtcagccc ttcaaggctt caactcgcca
     1081 ggaatgctgt cgctgggaca ggtgtcggcc tggcagcagc accacctagg acaagcagcc
     1141 ctcagctctc ttgttgctgg agggcagtta tctcagggtt ccaatttatc cattaatacc
     1201 aaccaaaaca tcagcatcaa gtccgaaccg atttcacctc ctcgggatcg tatgacccca
     1261 tcgggcttcc agcagcagca gcagcagcag cagcagcagc agccgccgcc accaccgcag
     1321 ccccagccac aacccccgca gccccagccc cgacaggaaa tggggcgctc ccctgtggac
     1381 agtctgagca gctctagtag ctcctatgat ggcagtgatc gggaggatcc acggggcgac
     1441 ttccattctc caattgtgct tggccgaccc ccaaacactg aggacagaga aagcccttct
     1501 gtaaagcgaa tgaggatgga cgcgtgggtt acctacccaa ctttcttgta caaagtggtt
     1561 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1621 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1681 tcacacgaga ggtcgtgttg ccg
//
