LOCUS       sequence-285585-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3014
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(488..512)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             520..561
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcaagatatc gcagcaaaac
       61 agcactccag gggtgaacgg aattagtgtt atccataccc aggcacatgc cagcggctta
      121 cagcaggttc ctcagctggt gcctgctggc cctgggggag gaggcaaagc tgtggctccc
      181 agcaagcaga gcaaaaagag ttcgcccatg gatcgaaaca gtgacgagta tcggcaacgc
      241 cgagagagga acaacatggc tgtgaaaaag agccggttga aaagcaagca gaaagcacaa
      301 gacacactgc agagagtcaa tcagctcaaa gaagagaatg aacggttgga agcaaaaatc
      361 aaattgctga ccaaggaatt aagtgtactc aaagatttgt ttcttgagca tgcacacaac
      421 cttgcagaca acgtacagtc cattagcact gaaaatacga cagcagatgg cgacaatgca
      481 ggacagtacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      541 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      601 tcttggcttt atatatcttg tggaaaggac gagccccggc tggcgtatcg ccagaa
//
