LOCUS       sequence-285591-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3016
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(770..794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             802..843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cggagccggg cgcctctccc
       61 gaggaccctt gggtcaaggt ggagtatgcc tacagcgaca acagcctgga ccccgggctt
      121 tttgtagaaa gcacccgcaa ggggagtgta gtgtccagag ctaatagcat cggttccacc
      181 agtgcctctt ctgtccccaa cacagatgat gaggacagtg attaccacca ggaggcctac
      241 aaggagtcct acaaagaccg gcggcggcgc gcacacactc aggctgagca gaagaggagg
      301 gacgccatca agagaggcta tgatgacctt cagaccatcg tccccacttg ccagcagcag
      361 gacttctcca ttggctccca aaagctcagc aaagccatcg ttctacaaaa gaccattgac
      421 tacattcagt ttttgcacaa ggagaagaaa aagcaggagg aggaggtgtc cacgttacgc
      481 aaggatgtca ccgccctaaa gatcatgaaa gtgaactatg agcagattgt gaaggcacac
      541 caggacaacc cccatgaagg ggaggaccag gtctctgacc aggtcaagtt caacgtgttt
      601 caaggcatca tggattccct gttccagtcc ttcaatgcct ccatctcagt ggccagcttc
      661 caggagctgt cagcatgtgt cttcagctgg atcgaggagc actgtaagcc tcagaccctg
      721 cgggagattg tgattggcgt cctgcaccaa ttgaaaaacc agctttactg cccaactttc
      781 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      841 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      901 tgtggaaagg acgaacgtaa tggagaaaga tacaatcc
//
