LOCUS       sequence-285597-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3018
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(683..707)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             715..756
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgacgcctca accctcgggt
       61 gcgcccactg tccaagtgac ccgtgagacg gagcggtcct tccccagagc ctcggaagac
      121 gaagtgacct gccccacgtc cgccccgccc agccccactc gcacacgggg gaactgcgca
      181 gaggcggaag agggaggctg ccgaggggcc ccgaggaagc tccgggcacg gcgcggggga
      241 cgcagccggc ctaagagcga gttggcactg agcaagcagc gacggagtcg gcgaaagaag
      301 gccaacgacc gcgagcgcaa tcgaatgcac aacctcaact cggcactgga cgccctgcgc
      361 ggtgtcctgc ccaccttccc agacgacgcg aagctcacca agatcgagac gctgcgcttc
      421 gcccacaact acatctgggc gctgactcaa acgctgcgca tagcggacca cagcttgtac
      481 gcgctggagc cgccggcgcc gcactgcggg gagctgggca gcccaggcgg ttcccccggg
      541 gactgggggt ccctctactc cccagtctcc caggctggca gcctgagtcc cgccgcgtcg
      601 ctggaggagc gacccgggct gctgggggcc accttttccg cctgcttgag cccaggcagt
      661 ctggctttct cagattttct gttgccaact ttcttgtaca aagtggttga tatcggtaag
      721 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgt ggccccggag
      841 cggctaattg g
//
