LOCUS       sequence-285600-        2435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3019
FEATURES             Location/Qualifiers
     source          1..2435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2267..2291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2299..2340
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga acaatccgtc agaaaccagt
       61 aaaccatcta tggagagtgg agatggcaac acaggcacac aaaccaatgg tctggacttt
      121 cagaagcagc ctgtgcctgt aggaggagca atctcaacag cccaggcgca ggctttcctt
      181 ggacatctcc atcaggtcca actcgctgga acaagtttac aggctgctgc tcagtcttta
      241 aatgtacagt ctaaatctaa tgaagaatcg ggggattcgc agcagccaag ccagccttcc
      301 cagcagcctt cagtgcaggc agccattccc cagacccagc ttatgctagc tggaggacag
      361 ataactgggc ttactttgac gcctgcccag caacagttac tactccagca ggcacaggca
      421 caggcacagc tgctggctgc tgcagtgcag cagcactccg ccagccagca gcacagtgct
      481 gctggagcca ccatctccgc ctctgctgcc acgcccatga cgcagatccc cctgtctcag
      541 cccatacaga tcgcacagga tcttcaacaa ctgcaacagc ttcaacagca gaatctcaac
      601 ctgcaacagt ttgtgttggt gcatccaacc accaatttgc agccagcgca gtttatcatc
      661 tcacagacgc cccagggcca gcagggtctc ctgcaagcgc aaaatcttct aacgcaacta
      721 cctcagcaaa gccaagccaa cctcctacag tcgcagccaa gcatcaccct cacctcccag
      781 ccagcaaccc caacacgcac aatagcagca accccaattc agacacttcc acagagccag
      841 tcaacaccaa agcgaattga tactcccagc ttggaggagc ccagtgacct tgaggagctt
      901 gagcagtttg ccaagacctt caaacaaaga cgaatcaaac ttggattcac tcagggtgat
      961 gttgggctcg ctatggggaa actatatgga aatgacttca gccaaactac catctctcga
     1021 tttgaagcct tgaacctcag ctttaagaac atgtgcaagt tgaagccact tttagagaag
     1081 tggctaaatg atgcagagaa cctctcatct gattcgtccc tctccagccc aagtgccctg
     1141 aattctccag gaattgaggg cttgagccgt aggaggaaga aacgcaccag catagagacc
     1201 aacatccgtg tggccttaga gaagagtttc ttggagaatc aaaagcctac ctcggaagag
     1261 atcactatga ttgctgatca gctcaatatg gaaaaagagg tgattcgtgt ttggttctgt
     1321 aaccgccgcc agaaagaaaa aagaatcaac ccaccaagca gtggtgggac cagcagctca
     1381 cctattaaag caattttccc cagcccaact tcactggtgg cgaccacacc aagccttgtg
     1441 actagcagtg cagcaactac cctcacagtc agccctgtcc tccctctgac cagtgctgct
     1501 gtgacgaatc tttcagttac aggcacttca gacaccacct ccaacaacac agcaaccgtg
     1561 atttccacag cgcctccagc ttcctcagca gtcacgtccc cctctctgag tccctcccct
     1621 tctgcctcag cctccacctc cgaggcatcc agtgccagtg agaccagcac aacacagacc
     1681 acctccactc ctttgtcctc ccctcttggg accagccagg tgatggtgac agcatcaggt
     1741 ttgcaaacag cagcagctgc tgcccttcaa ggagctgcac agttgccagc aaatgccagt
     1801 cttgctgcca tggcagctgc tgcaggacta aacccaagcc tgatggcacc ctcacagttt
     1861 gcggctggag gtgccttact cagtctgaat ccagggaccc tgagcggtgc tctcagccca
     1921 gctctaatga gcaacagtac actggcaact attcaagctc ttgcttctgg tggctctctt
     1981 ccaataacat cacttgatgc aactgggaac ctggtatttg ccaatgcggg aggagccccc
     2041 aacatcgtga ctgcccctct gttcctgaac cctcagaacc tctctctgct caccagcaac
     2101 cctgttagct tggtctctgc cgccgcagca tctgcaggga actctgcacc tgtagccagc
     2161 cttcacgcca cctccacctc tgctgagtcc atccagaact ctctcttcac agtggcctct
     2221 gccagcgggg ctgcgtccac caccaccacc gcctccaagg cacagtgccc aactttcttg
     2281 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2341 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2401 ggaaaggacg agggagactg catgaggctt gatta
//
