LOCUS       sequence-285606-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3021
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(758..782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             790..831
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cctctttgcc ctgccccctc
       61 cccggccggg acgcctccaa agctgtcttc ccagacctcg cccctgtccc gtcggtagcg
      121 gctgcctacc cgcttggctt gtcccctaca accgcagcct cccccaattt gtcctactcc
      181 aggccgtatg gccacctcct gtcttacccc tacaccgagc cagcgaaccc cggagactcc
      241 tacctgtcct gccagcaacc cgcggcgctc tctcagcccc tctgcggacc tgcagagcac
      301 cctcaggaac tcgaggcaga ctcggagaag ccgcggctgt ccccggaacc ctccgagcgg
      361 cgccctcagg cccccgccaa aaagctccgc aagccgagga ccatctactc cagcctgcag
      421 ctgcagcacc taaaccagcg tttccagcac acgcagtacc tggcgctgcc cgagagggcc
      481 cagctggcag cgcagctcgg cctcacccag acccaggtaa agatctggtt tcagaacaaa
      541 cgctccaagt ataagaagct cctgaagcag aattctgggg ggcaggaagg ggacttccct
      601 gggaggacct tctctgtgtc tccctgctcc ccacccctcc cctccctctg ggatctaccc
      661 aaggcaggga ccctgcccac cagtggctat ggcaacagct ttggagcctg gtatcagcat
      721 cactcctcag atgtcctggc ttcgcctcag atgatgtgcc caactttctt gtacaaagtg
      781 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      841 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      901 gatgtttggt ctgatgtgtg tgataa
//
