LOCUS       sequence-285609-        1595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3022
FEATURES             Location/Qualifiers
     source          1..1595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1427..1451)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1459..1500
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt atggaaatta ttctcacttc
       61 atgaagtttc ccgcaggcta tggaggctcc cctggccaca ctggctctac atccatgagc
      121 ccatcagcag ccttgtccac agggaagcca atggacagcc accccagcta cacagatacc
      181 ccagtgagtg ccccacggac tctgagtgca gtggggaccc ccctcaatgc cctgggctct
      241 ccatatcgag tcatcacctc tgccatgggc ccaccctcag gagcacttgc agcgcctcca
      301 ggaatcaact tggttgcccc acccagctct cagctaaatg tggtcaacag tgtcagcagt
      361 tcagaggaca tcaagccctt accagggctt cccgggattg gaaacatgaa ctacccatcc
      421 accagccccg gatctctggt taaacacatc tgtgccatct gtggagacag atcctcagga
      481 aagcactacg gggtatacag ttgtgaaggc tgcaaagggt tcttcaagag gacgataagg
      541 aaggacctca tctacacgtg tcgggataat aaagactgcc tcattgacaa gcgtcagcgc
      601 aaccgctgcc agtactgtcg ctatcagaag tgccttgtca tgggcatgaa gagggaagct
      661 gtgcaagaag aaagacagag gagccgagag cgagctgaga gtgaggcaga atgtgctacc
      721 agtggtcatg aagacatgcc tgtggagagg attctagaag ctgaacttgc tgttgaacca
      781 aagacagaat cctatggtga catgaatatg gagaactcga caaatgaccc tgttaccaac
      841 atatgtcatg ctgctgacaa gcagcttttc accctcgttg aatgggccaa gcgtattccc
      901 cacttctctg acctcacctt ggaggaccag gtcattttgc ttcgggcagg gtggaatgaa
      961 ttgctgattg cctctttctc ccaccgctca gtttccgtgc aggatggcat ccttctggcc
     1021 acgggtttac atgtccaccg gagcagtgcc cacagtgctg gggtcggctc catctttgac
     1081 agagtcctaa ctgagctggt ttccaaaatg aaagacatgc agatggacaa gtcggaactg
     1141 ggatgcctgc gagccattgt actctttaac ccagatgcca agggcctgtc caacccctct
     1201 gaggtggaga ctctgcgaga gaaggtttat gccacccttg aggcctacac caagcagaag
     1261 tatccggaac agccaggcag gtttgccaag ctgctgctgc gcctcccagc tctgcgttcc
     1321 attggcttga aatgcctgga gcacctcttc ttcttcaagc tcatcgggga cacccccatt
     1381 gacaccttcc tcatggagat gttggagacc ccgctgcaga tcacctgccc aactttcttg
     1441 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1501 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1561 ggaaaggacg atgagatggc gaaccgttgg aatag
//
