LOCUS       sequence-285612-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3023
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1055..1079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1087..1128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgttaacact accgtttgat
       61 gagtctgttg taatgccaga atcccagatg tgcagaaagt tttctagaga atgcgaggac
      121 cagaagcaaa ttaagaagcc agaaagcttt tccaaacaga ttgtccttcg aggaaagagc
      181 atcaaaaggg cccctggaga agaaaccgag aaagaagaag aggaggaaga cagggaagag
      241 gaagatgaaa atgggttgcc tagaaggagg ggtcttagga aaaaaaagac aacaaagctg
      301 cgattggaaa gggtcaagtt caggagacag gaagcgaacg cgcgcgagag gaacaggatg
      361 cacggcctca acgacgctct ggacaactta agaaaagtgg tcccctgtta ttctaaaacc
      421 cagaaactgt ccaaaataga gactttacga ctggccaaaa actacatctg ggcactttct
      481 gaaattctga gaatcggcaa gagaccagat ctgctcacat tcgtccaaaa cttatgcaaa
      541 ggtctttccc agccaactac aaacttggtg gcaggctgct tgcagctcaa cgccaggagt
      601 ttcctgatgg gtcagggtgg ggaggctgca caccacacaa ggtcacccta ctctaccttc
      661 tacccaccct accacagccc tgagctcacc actcccccag ggcatgggac tcttgataat
      721 tccaagtcca tgaaacccta caattattgc agtgcgtatg aatccttcta tgaaagtact
      781 tcccctgagt gtgccagccc tcagtttgaa ggtcccttaa gtcctccccc aattaactat
      841 aatgggatat tttccctgaa gcaagaagaa accttggact atggtaaaaa ttacaattac
      901 ggcatgcatt actgtgcagt gccacccagg ggtccccttg ggcagggtgc catgttcagg
      961 ttgcccaccg acagccactt cccttacgac ttacatctgc gcagccaatc tctcacaatg
     1021 caagatgaat taaatgcagt ttttcataat tagaacccag ctttcttgta caaagtggtt
     1081 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1141 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1201 caggattgtc cctacacaaa tat
//
