LOCUS       sequence-285615-        1217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3024
FEATURES             Location/Qualifiers
     source          1..1217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1049..1073)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1081..1122
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agcgcccctg cgaggagacg
       61 acctccgaga gcgacatgga cgagaccatc gacgtgggga gcgagaacaa ttactcgggg
      121 caaagtacta gctctgtgat tagattgaat tctccaacaa caacatctca gattatggca
      181 agaaagaaaa ggagagggat tatagagaaa aggcgtcggg atcggataaa taacagttta
      241 tctgagttga gaagacttgt gccaactgct tttgaaaaac aaggatctgc aaagttagaa
      301 aaagctgaaa tattgcaaat gacagtggat catttgaaga tgcttcaggc aacagggggt
      361 aaaggctact ttgacgcaca cgctcttgcc atggacttca tgagcatagg attccgagag
      421 tgcctaacag aagttgcgcg gtacctgagc tccgtggaag gcctggactc ctcggatccg
      481 ctgcgggtgc ggcttgtgtc tcatctcagc acttgcgcca cccagcggga ggcggcggcc
      541 atgacatcct ccatggccca ccaccatcat ccgctccacc cgcatcactg ggccgccgcc
      601 ttccaccacc tgcccgcagc cctgctccag cccaacggcc tccatgcctc agagtcaacc
      661 ccttgtcgcc tctccacaac ttcagaagtg cctcctgccc acggctctgc tctcctcacg
      721 gccacgtttg cccatgcgga ttcagccctc cgaatgccat ccacgggcag cgtcgccccc
      781 tgcgtgccac ctctctccac ctctctcttg tccctctctg ccaccgtcca cgccgcagcc
      841 gcagcagcca ccgcggctgc acacagcttc cctctgtcct tcgcgggggc attccccatg
      901 cttcccccaa acgcagcagc agcagtggcc gcggccacag ccatcagccc gcccttgtca
      961 gtatcagcca cgtccagtcc tcagcagacc agcagtggaa caaacaataa accttaccga
     1021 ccctggggga cagaagttgg agctttttac ccaactttct tgtacaaagt ggttgatatc
     1081 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1141 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagcagcca
     1201 aagcaaggat gcccgga
//
