LOCUS       sequence-285618-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3025
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(582..593)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(764..788)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             796..837
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgatggacct ttttgaaact
       61 ggctcctatt tcttctactt ggatggggaa aatgttactc tgcagccatt agaagtggca
      121 gaaggctctc ctttgtatcc agggagtgat ggtaccttgt ccccctgcca ggaccaaatg
      181 cccccggaag cggggagcga cagcagcgga gaggaacatg tcctggcgcc cccgggcctg
      241 cagcctccac actgccccgg ccagtgtctg atctgggctt gcaagacctg caagagaaaa
      301 tctgccccca ctgaccggcg aaaagccgcc accctgcgcg aaaggaggag gctaaagaaa
      361 atcaacgagg ccttcgaggc actgaagcgg cgaactgtgg ccaaccccaa ccagaggctg
      421 cccaaggtgg agattctgcg gagcgccatc agctatattg agcggctgca ggacctgctg
      481 caccggctgg atcagcagga gaagatgcag gagctggggg tggacccctt cagctacaga
      541 cccaaacaag aaaatcttga gggtgcggat ttcctgcgca cctgcagctc ccagtggcca
      601 agtgtttccg atcattccag ggggctcgtg ataacggcta aggaaggagg agcaagtatt
      661 gattcgtcag cctcgagtag ccttcgatgc ctttcttcca tcgtggacag tatttcctcg
      721 gaggaacgca aactcccctg cgtggaggaa gtggtggaga agtacccaac tttcttgtac
      781 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
      841 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      901 aaggacgagc cctaagtttt tcagtggagc ga
//
