LOCUS       sequence-285621-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3026
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1061..1085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1093..1134
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccatggaatc tggagccgag
       61 aaccagcaga gtggagatgc agctgtaaca gaagctgaaa accaacaaat gacagttcaa
      121 gcccagccac agattgccac attagcccag gtatctatgc cagcagctca tgcaacatca
      181 tctgctccca ccgtaactct agtacagctg cccaatgggc agacagttca agtccatgga
      241 gtcattcagg cggcccagcc atcagttatt cagtctccac aagtccaaac agttcagtct
      301 tcctgtaagg acttaaaaag acttttctcc ggaacacaga tttcaactat tgcagaaagt
      361 gaagattcac aggagtcagt ggatagtgta actgattccc aaaagcgaag ggaaattctt
      421 tcaaggaggc cttcctacag gaaaattttg aatgacttat cttctgatgc accaggagtg
      481 ccaaggattg aagaagagaa gtctgaagag gagacttcag cacctgccat caccactgta
      541 acggtgccaa ctccaattta ccaaactagc agtggacagt atattgccat tacccaggga
      601 ggagcaatac agctggctaa caatggtacc gatggggtac agggcctgca aacattaacc
      661 atgaccaatg cagcagccac tcagccgggt actaccattc tacagtatgc acagaccact
      721 gatggacagc agatcttagt gcccagcaac caagttgttg ttcaagctgc ctctggagac
      781 gtacaaacat accagattcg cacagcaccc actagcacta ttgcccctgg agttgttatg
      841 gcatcctccc cagcacttcc tacacagcct gctgaagaag cagcacgaaa gagagaggtc
      901 cgtctaatga agaacaggga agcagctcga gagtgtcgta gaaagaagaa agaatatgtg
      961 aaatgtttag aaaacagagt ggcagtgctt gaaaatcaaa acaagacatt gattgaggag
     1021 ctaaaagcac ttaaggacct ttactgccac aaatcagatt acccaacttt cttgtacaaa
     1081 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1141 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1201 gacgaggtgt gtatcctcga caattcggg
//
