LOCUS       sequence-285624-        1337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3027
FEATURES             Location/Qualifiers
     source          1..1337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1169..1193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1201..1242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgctaaccgc tgtctgcggc
       61 tctctgggca gccagcacac ggaagcgccg cacgcctccc cgccgcgcct cgacctgcag
      121 cctctccaaa cttaccaggg ccacacgagc cctgaggccg gggactaccc ctccccgctg
      181 cagcctggag agctgcagag cctcccgctg ggcccggagg tggacttctc gcagggctat
      241 gagctgccag gggcctcctc gcgggtaacc tgcgaggacc tggaaagcga cagtcccttg
      301 gccccgggcc ccttttccaa gctcctgcag ccggacatgt cacaccatta tgaatcgtgg
      361 ttcaggccga ctcacccagg cgcggaggat ggctcgtggt gggaccttca tccgggcacc
      421 agctggatgg acctccccca cactcagggc gcgctgacct cacctggcca cccgggggcg
      481 cttcaggcgg gcttgggggg ctacgtcgga gaccaccagc tttgtgcccc gccaccccac
      541 ccgcatgcgc accacctcct tccagctgcc ggagggcagc atctcctagg gccgcccgac
      601 ggggctaagg ccttggaagt agccgccccg gagtctcaag ggctggattc cagcctggac
      661 ggggcggcgc gtcccaaagg ctcccggcgg tcggtgcccc gcagctcagg ccagaccgtc
      721 tgtcgctgcc ccaactgtct ggaggcggag cgactggggg ctccatgtgg gcccgatggg
      781 ggcaagaaga agcatttgca caactgccac atcccgggct gcgggaaagc ctacgccaag
      841 acgtcgcacc tgaaggcgca cctgcgctgg cacagcggcg accgtccctt cgtgtgcaac
      901 tggctcttct gcggcaagcg cttcacgcgc tcggacgagc tgcagcgcca cctccagacc
      961 cacaccggca ccaagaagtt cccctgtgca gtctgcagcc gcgtcttcat gcgcagcgac
     1021 cacctggcca agcacatgaa aacccacgag ggcgccaagg aggaggcggc tggggcggcc
     1081 tcgggagagg gcaaggccgg cggcgcagtg gagccccccg ggggcaaagg caaacgcgag
     1141 gccgagggca gcgtggctcc ctccaacttg ccaactttct tgtacaaagt ggttgatatc
     1201 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1261 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatagggtt
     1321 tgaccttcgt cgagagg
//
