LOCUS       sequence-285630-        1268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3029
FEATURES             Location/Qualifiers
     source          1..1268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1100..1124)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1132..1173
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cccgcctgct gcatgcagaa
       61 gagtgggctg aagtgaagga gttgggagac caccatcgcc agccccagcc gcatcatctc
      121 ccgcaaccgc cgccgccgcc gcagccacct gcaactttgc aggcgagaga gcatcccgtc
      181 tacccgcctg agctgtccct cctggacagc accgacccac gcgcctggct ggctcccact
      241 ttgcagggca tctgcacggc acgcgccgcc cagtatttgc tacattcccc ggagctgggt
      301 gcctcagagg ccgctgcgcc ccgggacgag gtggacggcc ggggggagct ggtaaggagg
      361 agcagcggcg gtgccagcag cagcaagagc cccgggccgg tgaaagtgcg ggaacagctg
      421 tgcaagctga aaggcggggt ggtggtagac gagctgggct gcagccgcca acgggcccct
      481 tccagcaaac aggtgaatgg ggtgcagaag cagagacggc tagcagccaa cgccagggag
      541 cggcgcagga tgcatgggct gaaccacgcc ttcgaccagc tgcgcaatgt tatcccgtcg
      601 ttcaacaacg acaagaagct gtccaaatat gagaccctgc agatggccca aatctacatc
      661 aacgccttgt ccgagctgct acaaacgccc agcggagggg aacagccacc gccgcctcca
      721 gcctcctgca aaagcgacca ccaccacctt cgcaccgcgg cctcctatga agggggcgcg
      781 ggcaacgcga ccgcagctgg ggctcagcag gcttccggag ggagccagcg gccgaccccg
      841 cccgggagtt gccggactcg cttctcagcc ccagcttctg cgggagggta ctcggtgcag
      901 ctggacgctc tgcacttctc gactttcgag gacagcgccc tgacagcgat gatggcgcaa
      961 aagaatttgt ctccttctct ccccgggagc atcttgcagc cagtgcagga ggaaaacagc
     1021 aaaacttcgc ctcggtccca cagaagcgac ggggaatttt ccccccattc ccattacagt
     1081 gactcggatg aggcaagtta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1141 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1201 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagcgtgt agacgagatg
     1261 ttgacgcc
//
