LOCUS       sequence-285633-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3030
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1664..1688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1696..1737
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt gggagcacag gaaagagccc
       61 tggaccatag aaagccaagt acgagtagca agaaaaccaa aagggtggga atggatcaaa
      121 ggtgtgaaaa cagatctgtc tcgtaactgt gtaatcaagg aactagcacc acaacaggaa
      181 ggtaacccag gagaagtatt ccacacagtg acattggaac aacatgaaaa acatgacatt
      241 gaagagtttt gcttcaggga aatcaagaaa aaaatacacg actttgactg tcagtggaga
      301 gatgatgaaa gaaattgcaa caaagtgact acggccccaa aagaaaatct tacttgtagg
      361 agagaccaac gcgatagaag aggtatagga aacaagtcta ttaaacatca gcttggatta
      421 agctttctac cacatcccca tgaactgcag cagtttcaag ctgaagggaa aatttatgaa
      481 tgtaaccatg ttgagaagtc tgtcaaccat ggttcctcag tttcaccacc ccaaataatt
      541 tcttctacca tcaaaaccca tgtttctaat aaatatggga ctgatttcat ctgttcttca
      601 ttactcacac aagaacagaa atcatgcatt agggaaaaac cttacagata tattgagtgc
      661 gacaaagcct tgaatcatgg ctcacacatg actgtacgtc aggtaagtca ttctggagag
      721 aaaggatata aatgtgatct gtgtggcaag gtctttagtc aaaaatcaaa ccttgcgcgt
      781 cattggagag ttcatactgg agagaaacca tacaaatgta atgaatgtga cagaagtttc
      841 agtcgcaact catgccttgc actacatcgg agagttcaca ctggagagaa accttacaaa
      901 tgttatgagt gtgacaaggt cttcagtcga aattcatgcc ttgcactaca tcagaaaact
      961 catattggag agaaacctta cacatgtaaa gagtgtggca aagcctttag tgtgaggtca
     1021 acacttacca accatcaggt aattcatagt ggcaagaaac cttacaaatg caatgaatgt
     1081 ggcaaggtgt tcagtcagac ttcaagcctt gcaactcatc agagaattca cactggggag
     1141 aaaccataca agtgtaatga atgtggtaaa gtcttcagtc agacttcaag ccttgcaagg
     1201 cattggagaa ttcatactgg agagaaacct tacaaatgca atgaatgtgg taaggttttc
     1261 agttacaatt cacaccttgc gagtcatcgg agagttcata ctggagagaa accttacaag
     1321 tgtaatgagt gtgggaaagc ctttagtgtg cattcgaact taactaccca tcaggtcatc
     1381 catactggag agaagcctta caaatgtaat caatgtggca aaggcttcag tgtgcattca
     1441 agcctaacta cccatcaggt catccatact ggagaaaaac cttacaaatg taatgagtgt
     1501 ggcaaatcct ttagtgtgcg cccaaacctc actagacatc agataatcca tactggaaag
     1561 aaaccttaca aatgtagtga ttgtgggaag tcctttagtg tgcgcccaaa cctcttcaga
     1621 catcaaatta tccatactaa ggagaaacct tataaaagaa attacccaac tttcttgtac
     1681 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1741 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1801 aaggacgact ccgcaagcta ggattttgta ac
//
