LOCUS       sequence-285639-        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3032
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1103..1127)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1135..1176
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cggtgaatta cgcggcgggg
       61 ctgtcgccgt acgcggacaa gggcaagtgc ggcctcccgg agatcttcga ccccccggag
      121 gagctggagc ggaaggtgtg ggaactggcg aggctggtct ggcagtcttc cagtgtggtg
      181 ttccacacgg gtgccggcat cagcactgcc tctggcatcc ccgacttcag gggtccccac
      241 ggagtctgga ccatggagga gcgaggtctg gcccccaagt tcgacaccac ctttgagagc
      301 gcgcggccca cgcagaccca catggcgctg gtgcagctgg agcgcgtggg cctcctccgc
      361 ttcctggtca gccagaacgt ggacgggctc catgtgcgct caggcttccc cagggacaaa
      421 ctggcagagc tccacgggaa catgtttgtg gaagaatgtg ccaagtgtaa gacgcagtac
      481 gtccgagaca cagtcgtggg caccatgggc ctgaaggcca cgggccggct ctgcaccgtg
      541 gctaaggcaa gggggctgcg agcctgcagg ggagagctga gggacaccat cctagactgg
      601 gaggactccc tgcccgaccg ggacctggca ctcgccgatg aggccagcag gaacgccgac
      661 ctgtccatca cgctgggtac atcgctgcag atccggccca gcgggaacct gccgctggct
      721 accaagcgcc ggggaggccg cctggtcatc gtcaacctgc agcccaccaa gcacgaccgc
      781 catgctgacc tccgcatcca tggctacgtt gacgaggtca tgacccggct catgaagcac
      841 ctggggctgg agatccccgc ctgggacggc ccccgtgtgc tggagagggc gctgccaccc
      901 ctgccccgcc cgcccacccc caagctggag cccaaggagg aatctcccac ccggatcaac
      961 ggctctatcc ccgccggccc caagcaggag ccctgcgccc agcacaacgg ctcagagccc
     1021 gccagcccca aacgggagcg gcccaccagc cctgcccccc acagaccccc caaaagggtg
     1081 aaggccaagg cggtccccag ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1141 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1201 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagca attgcagttc
     1261 tacactcact c
//
