LOCUS       sequence-285642-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3033
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(989..1013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1021..1062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tccaggcggc cgagcgcccc
       61 caggagtggg ccatggaggg cccccgcgac gggctgaaga aggagcggct actggacgac
      121 cgccacgaca gcggcctgga ctccatgaaa gacgaggagt acgagcagat ggtcaaggag
      181 ctgcaggaga tccgcctcga gccgcaggag gtgccgcgcg gctcggagcc ctggaagcag
      241 cagctcaccg aggacgggga ctcgttcctg cacttggcca tcatccatga agaaaaggca
      301 ctgaccatgg aagtgatccg ccaggtgaag ggagacctgg ccttcctcaa cttccagaac
      361 aacctgcagc agactccact ccacttggct gtgatcacca accagccaga aattgctgag
      421 gcacttctgg gagctggctg tgatcctgag ctccgagact ttcgaggaaa taccccccta
      481 caccttgcct gtgagcaggg ctgcctggcc agcgtgggag tcctgactca gtcctgcacc
      541 accccgcacc tccactccat cctgaaggct accaactaca atggccacac gtgtctacac
      601 ttagcctcta tccatggcta cctgggcatc gtggagcttt tggtgtcctt gggtgctgat
      661 gtcaatgctc aggagccctg taatggccgg actgcccttc acctcgcagt ggacctgcaa
      721 aatcctgacc tggtgtcact cctgttgaag tgtggggctg atgtcaacag agttacctac
      781 cagggctatt ctccctacca gctcacctgg ggccgcccaa gcacccggat acagcagcag
      841 ctgggccagc tgacactaga aaaccttcag atgctgccag agagtgagga tgaggagagc
      901 tatgacacag agtcagagtt cacggagttc acagaggacg agctgcccta tgatgactgt
      961 gtgtttggag gccagcgtct gacgttatgc ccaactttct tgtacaaagt ggttgatatc
     1021 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1081 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatacacac
     1141 ccttcttagc cattcat
//
