LOCUS       sequence-285645-        1268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3034
FEATURES             Location/Qualifiers
     source          1..1268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             910..939
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1100..1124)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1132..1173
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgtcttacct caaacaaccc
       61 ccatacggca tgaacgggct gggcctggcc gggcccgcca tggacctcct gcacccatcc
      121 gtgggctatc cggccactcc gcggaagcag cggcgggagc gcaccacctt cacgcgttca
      181 cagctggacg tgctcgaggc gctcttcgcc aagactcgct accctgacat cttcatgcgg
      241 gaggaggtgg cgctcaagat caacctgccg gagtctagag tccaggtctg gttcaagaac
      301 cgccgcgcca aatgccgcca gcagcagcag agcgggagcg gaaccaagag ccgcccagcc
      361 aagaagaagt cctctccagt gcgggagagc tcgggctccg aaagcagtgg ccaattcacg
      421 ccgccagctg tgtccagctc tgcctcgtcc tctagctcgg cgtccagctc ttccgccaac
      481 ccagcggctg cagcggctgc gggactaggt gggaacccgg tggcggccgc gtcgtcgctg
      541 agtacaccag ctgcctcatc tatctggagc ccggcctcca tctcgccagg ctcagcgccc
      601 gcgtccgtgt cggtgccgga gccattggcc gcgcctagca acacctcgtg tatgcagcgc
      661 tccgtagctg caggcgccgc caccgcagca gcctcttatc ccatgtccta cggccagggc
      721 ggcagctacg gccaaggcta ccctacgccc tcctcttcct actttggcgg cgtggactgc
      781 agctcatacc tagcgcccat gcactcacat caccacccgc accagctgag ccccatggca
      841 ccctcctcca tggcgggcca ccatcatcac cacccacatg cgcaccaccc gttgagccag
      901 tcctcaggcc accaccacca ccatcaccac caccaccacc aaggctacgg tggctctggg
      961 cttgccttca actctgccga ctgcttggat tacaaggagc ctggcgccgc tgctgcttcc
     1021 tccgcctgga aactcaactt caactccccc gactgtctgg actataagga ccaagcctca
     1081 tggcggttcc aggtcttgtg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1141 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1201 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagtagat cggtagtcgg
     1261 gctaacac
//
