LOCUS       sequence-285648-        1274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3035
FEATURES             Location/Qualifiers
     source          1..1274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1106..1130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1138..1179
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctggggtcgc gtgcttggga
       61 aaagctgccg acgcagatga atggtgcgac agcggcctgg gctccctggg tccggacgca
      121 gcggcccccg gaggacctgg gttgggcgcg gagttgggcc cggggctgtc gtgggctccc
      181 ctcgtcttcg gctacgtcac tgaggatggg gacacggcac tgcacttggc tgtgattcat
      241 cagcatgaac ccttcctgga ttttcttcta ggcttctcgg ccggcactga gtacatggac
      301 ctgcagaatg acctaggcca gacagccctg cacctggcag ccatcctggg ggagacatcc
      361 acggtggaga agctgtacgc agcaggcgcc gggctgtgtg tggcggagcg taggggccac
      421 acggcgctgc acctggcctg ccgtgtgggg gcacacgcct gtgcccgtgc cctgcttcag
      481 ccccgccccc ggcgccccag ggaagccccc gacacctacc tcgctcaggg ccctgaccgt
      541 actcccgaca ccaaccatac ccctgtcgcc ttgtaccccg attccgactt ggagaaggaa
      601 gaagaggaga gtgaggagga ctggaagctg cagctggagg ctgaaaacta cgagggccac
      661 accccactcc acgtggccgt tatccacaaa gatgtggaga tggtccggct gctccgagat
      721 gctggagctg accttgacaa accggagccc acgtgcggcc ggagccccct tcatttggca
      781 gtggaggccc aggcagccga tgtgctggag cttctcctga gggcaggcgc gaaccctgct
      841 gcccgcatgt acggtggccg caccccactc ggcagtgcca tgctccggcc caaccccatc
      901 ctcgcccgcc tcctccgtgc acacggagcc cctgagcccg agggcgagga cgagaaatcc
      961 ggcccctgca gcagcagtag cgacagcgac agcggagacg agggcgatga atacgacgac
     1021 attgtggttc acagcagccg cagccaaacc cggctgcctc ccaccccagc ctcaaaacct
     1081 cttcctgacg acccccgccc cgtgtgccca actttcttgt acaaagtggt tgatatcggt
     1141 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1201 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga taaactctgg
     1261 atcggattac ctcc
//
