LOCUS       sequence-285651-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3036
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             840..857
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(1091..1115)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1123..1164
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccgaaatgag cttcctgagc
       61 agcgaggtgt tggtggggga cttgatgtcc cccttcgacc agtcgggttt gggggctgaa
      121 gaaagcctag gtctcttaga tgattacctg gaggtggcca agcacttcaa acctcatggg
      181 ttctccagcg acaaggctaa ggcgggctcc tccgaatggc tggctgtgga tgggttggtc
      241 agtccctcca acaacagcaa ggaggatgcc ttctccggga cagattggat gttggagaaa
      301 atggatttga aggagttcga cttggatgcc ctgttgggta tagatgacct ggaaaccatg
      361 ccagatgacc ttctgaccac gttggatgac acttgtgatc tctttgcccc cctagtccag
      421 gagactaata agcagccccc ccagacggtg aacccaattg gccatctccc agaaagttta
      481 acaaaacccg accaggttgc ccccttcacc ttcttacaac ctcttcccct ttccccaggg
      541 gtcctgtcct ccactccaga tcattccttt agtttagagc tgggcagtga agtggatatc
      601 actgaaggag ataggaagcc agactacact gcttacgttg ccatgatccc tcagtgcata
      661 aaggaggaag acaccccttc agataatgat agtggcatct gtatgagccc agagtcctat
      721 ctggggtctc ctcagcacag cccctctacc aggggctctc caaataggag cctcccatct
      781 ccaggtgttc tctgtgggtc tgcccgtccc aaaccttacg atcctcctgg agagaagatg
      841 gtagcagcaa aagtaaaggg tgagaaactg gataagaagc tgaaaaaaat ggagcaaaac
      901 aagacagcag ccactaggta ccgccagaag aagagggcgg agcaggaggc tcttactggt
      961 gagtgcaaag agctggaaaa gaagaacgag gctctaaaag agagggcgga ttccctggcc
     1021 aaggagatcc agtacctgaa agatttgata gaagaggtcc gcaaggcaag ggggaagaaa
     1081 agggtcccct acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1141 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1201 atttcttggc tttatatatc ttgtggaaag gacgacagac tagaggggga ggcgaccta
//
