LOCUS       sequence-285654-        1253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3037
FEATURES             Location/Qualifiers
     source          1..1253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1085..1109)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1117..1158
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cggtggaaag gatgcgcatg
       61 cgcccgtggc tggaggagca gataaactcc aacacgatcc cggggctcaa gtggcttaac
      121 aaggaaaaga agatttttca gatcccctgg atgcatgcgg ctagacatgg gtgggatgtg
      181 gaaaaagatg caccactctt tagaaactgg gcaatccata caggaaagca tcaaccagga
      241 gtagataaac ctgatcccaa aacatggaag gcgaatttca gatgcgccat gaattccttg
      301 cctgatattg aagaagtcaa ggataaaagc ataaagaaag gaaataatgc cttcagggtc
      361 taccgaatgc tgcccctatc agaacggcct tctaagaaag gaaagaaacc aaagacagaa
      421 aaagaagaca aagttaagca catcaagcaa gaaccagttg agtcatctct ggggcttagt
      481 aatggagtaa gtgatctttc tcctgagtat gcggtcctga cttcaactat aaaaaatgaa
      541 gtggatagta cggtgaacat catagttgta ggacagtccc atctggacag caacattgag
      601 aatcaagaga ttgtcaccaa tccgccagac atttgccaag ttgtagaggt gaccactgag
      661 agcgacgagc agccggtcag catgagcgag ctctaccctc tgcagatctc ccccgtgtct
      721 tcctatgcag aaagcgaaac gactgatagt gtgcccagcg atgaagagag tgccgagggg
      781 cggccacact ggcggaagag gaatattgaa ggcaaacagt acctcagcaa catggggact
      841 cgaggctcct acctgctgcc cggcatggcg tccttcgtca cttccaacaa accggacctc
      901 caggtcacca tcaaagagga gagcaatccg gtgccttaca acagctcctg gccccctttt
      961 caagacctcc ccctttcttc ctccatgacc ccagcatcca gcagcagtcg gccagaccgg
     1021 gagacccggg ccagcgtcat caagaaaaca tcggatatca cccaggcccg cgtcaagagc
     1081 tgttacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1141 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1201 tggctttata tatcttgtgg aaaggacgag agtataacct agccaaccta agc
//
