LOCUS       sequence-285657-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3038
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1091..1115)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1123..1164
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgccggtgga aaggatgcgc
       61 atgcgcccgt ggctggagga gcagataaac tccaacacga tcccggggct caagtggctt
      121 aacaaggaaa agaagatttt tcagatcccc tggatgcatg cggctagaca tgggtgggat
      181 gtggaaaaag atgcaccact ctttagaaac tgggcaatcc atacaggaaa gcatcaacca
      241 ggagtagata aacctgatcc caaaacatgg aaggcgaatt tcagatgcgc catgaattcc
      301 ttgcctgata ttgaagaagt caaggataaa agcataaaga aaggaaataa tgccttcagg
      361 gtctaccgaa tgctgcccct atcagaacgg ccttctaaga aaggaaagaa accaaagaca
      421 gaaaaagaag acaaagttaa gcacatcaag caagaaccag ttgagtcatc tctggggctt
      481 agtaatggag taagtgatct ttctcctgag tatgcggtcc tgacttcaac tataaaaaat
      541 gaagtggata gtacggtgaa catcatagtt gtaggacagt cccatctgga cagcaacatt
      601 gagaatcaag agattgtcac caatccgcca gacatttgcc aagttgtaga ggtgaccact
      661 gagagcgacg agcagccggt cagcatgagc gagctctacc ctctgcagat ctcccccgtg
      721 tcttcctatg cagaaagcga aacgactgat agtgtgccca gcgatgaaga gagtgccgag
      781 gggcggccac actggcggaa gaggaatatt gaaggcaaac agtacctcag caacatgggg
      841 actcgaggct cctacctgct gcccggcatg gcgtccttcg tcacttccaa caaaccggac
      901 ctccaggtca ccatcaaaga ggagagcaat ccggtgcctt acaacagctc ctggccccct
      961 tttcaagacc tccccctttc ttcctccatg accccagcat ccagcagcag tcggccagac
     1021 cgggagaccc gggccagcgt catcaagaaa acatcggata tcacccaggc ccgcgtcaag
     1081 agctgttaga acccagcttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1141 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1201 atttcttggc tttatatatc ttgtggaaag gacgaccatt gtaacaccca aatcccgca
//
