LOCUS       sequence-285660-        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3039
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1276..1317
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cccactctgc tgggggtact
       61 gcaggggtgg ggctggaggc tgcagagccc acagccctgc tcaccagggc agagccccct
      121 tcagaaccca cagagatccg tccacaaaag cggaaaaagg ggccagcccc caaaatgctg
      181 gggaacgagc tatgcagcgt gtgtggggac aaggcctcgg gcttccacta caatgttctg
      241 agctgcgagg gctgcaaggg attcttccgc cgcagcgtca tcaagggagc gcactacatc
      301 tgccacagtg gcggccactg ccccatggac acctacatgc gtcgcaagtg ccaggagtgt
      361 cggcttcgca aatgccgtca ggctggcatg cgggaggagt gtgtcctgtc agaagaacag
      421 atccgcctga agaaactgaa gcggcaagag gaggaacagg ctcatgccac atccttgccc
      481 cccagggctt cctcaccccc ccaaatcctg ccccagctca gcccggaaca actgggcatg
      541 atcgagaagc tcgtcgctgc ccagcaacag tgtaaccggc gctccttttc tgaccggctt
      601 cgagtcacgc cttggcccat ggcaccagat ccccatagcc gggaggcccg tcagcagcgc
      661 tttgcccact tcactgagct ggccatcgtc tctgtgcagg agatagttga ctttgctaaa
      721 cagctacccg gcttcctgca gctcagccgg gaggaccaga ttgccctgct gaagacctct
      781 gcgatcgagg tgatgcttct ggagacatct cggaggtaca accctgggag tgagagtatc
      841 accttcctca aggatttcag ttataaccgg gaagactttg ccaaagcagg gctgcaagtg
      901 gaattcatca accccatctt cgagttctcc agggccatga atgagctgca actcaatgat
      961 gccgagtttg ccttgctcat tgctatcagc atcttctctg cagaccggcc caacgtgcag
     1021 gaccagctcc aggtagagag gctgcagcac acatatgtgg aagccctgca tgcctacgtc
     1081 tccatccacc atccccatga ccgactgatg ttcccacgga tgctaatgaa actggtgagc
     1141 ctccggaccc tgagcagcgt ccactcagag caagtgtttg cactgcgtct gcaggacaaa
     1201 aagctcccac cgctgctctc tgagatctgg gatgtgcacg aatgcccaac tttcttgtac
     1261 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1321 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1381 aaggacgaag tgtaaccgga tttcccaaca ca
//
