LOCUS       sequence-285663-        1625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3040
FEATURES             Location/Qualifiers
     source          1..1625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1457..1481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1489..1530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaacaattc ccagggaaga
       61 gtgaccttcg aggatgtcac tgtgaacttc acccaggggg agtggcagcg gctgaatccc
      121 gaacagagaa acttgtacag ggatgtgatg ctggagaatt acagcaacct tgtctctgtg
      181 ggacaagggg aaaccaccaa acccgatgtg atcttgaggt tggaacaagg aaaggagcca
      241 tggttggagg aagaggaagt gctgggaagt ggccgtgcag aaaaaaatgg ggacattgga
      301 gggcagattt ggaagccaaa ggatgtgaaa gagagtctcg caagagaagt cccatcaatc
      361 aataaggaaa cgctgactac gcagaaaggt gtagaatgtg acggatctaa gaaaatactt
      421 ccactgggca tagatgatgt atcttccttg caacactatg tacaaaataa ttctcacgat
      481 gataatggat acagaaaatt agttggcaat aatccatcca aatttgtagg acaacaactg
      541 aaatgtaatg cctgtagaaa gttattcagt tcaaagtcac gccttcaaag tcacctgagg
      601 aggcatgcct gtcaaaaacc ctttgaatgt catagctgtg gaagagcatt cggggagaag
      661 tggaagcttg ataaacatca gaaaactcac gcagaggaaa ggccctataa atgtgagaac
      721 tgtggaaatg cctacaagca gaagtcaaat ctctttcaac atcagaaaat gcatactaaa
      781 gagaaaccct atcagtgtaa gacatgtgga aaagcctttt cctggaaatc atcctgcatt
      841 aatcatgaga aaattcataa tgccaagaaa tcctatcagt gtaatgaatg tgagaaatcc
      901 ttcaggcaga actcaaccct cattcaacat aaaaaagttc acactggaca aaaacccttt
      961 caatgtacgg actgtggaaa ggctttcatt tacaagtcag atcttgtgaa acaccagaga
     1021 atacacacgg gagagaaacc ctataaatgt agcatatgtg agaaggcctt ttcccagaaa
     1081 tccaatgtca tcgatcatga gaaaattcac actgggaaga gagcttatga gtgtgatcta
     1141 tgtggaaata cctttatcca gaagaaaaac ctcattcaac ataaaaaaat ccatactggg
     1201 gaaaagccct atgaatgtaa cagatgtgga aaagccttct ttcagaagtc aaaccttcat
     1261 agccatcaga aaactcatag cggagagagg acctatagat gtagtgaatg tggaaaaacc
     1321 ttcatccgga aattaaacct tagtttgcat aaaaaaaccc atactggaca aaaaccttat
     1381 ggatgttctg aatgcggtaa agccttcgct gacaggtcat accttgttag gcaccagaaa
     1441 agaattcact ccagattgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1501 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1561 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtacgctt atagacccaa
     1621 tacca
//
