LOCUS       sequence-285666-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3041
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(737..761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             769..810
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgctaccagg ctcctcacag
       61 gaccaggcct ggagcctgga gcctccggct gccacggctg ctgcctcctc atcttcggga
      121 ccccaggagc gggagggcgc tgggagcccc gcggcccccg ggacgctgcc cctggagaag
      181 gtgaagcggc cgatgaacgc gttcatggtg tggagctccg ctcagcgccg ccagatggcg
      241 cagcagaacc ccaagatgca caactccgag atctccaagc gcctgggcgc gcagtggaag
      301 ctgctggacg aggacgagaa gcggcccttc gtggaggagg ccaagcggct ccgcgcccga
      361 cacctgcgcg actaccccga ctacaagtac cggcctcggc gcaaggccaa gagctcgggc
      421 gccggacctt cccgctgcgg acagggaaga ggcaacctgg ccagcggcgg cccgctctgg
      481 gggccggggt acgcgaccac ccaaccgagc agaggctttg ggtacagacc ccccagctac
      541 tcgacagcct acctgcctgg cagctatggc tcttcccact gcaaactgga agccccctca
      601 ccgtgctccc tccctcagag tgaccctagg ctccaggggg aactgctgcc cacctatacc
      661 cactacctgc cccctggctc tcccactcca tacaaccctc cccttgctgg tgcccccatg
      721 cccctaaccc acctctaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      781 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      841 atttcgattt cttggcttta tatatcttgt ggaaaggacg atagagtttc taatttaaaa
      901 tttcg
//
