LOCUS       sequence-285669-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3042
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(830..854)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             862..903
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agtctggcaa gacggcttct
       61 cccaagagca tgccgaaaga tgcacagatg atggcacaaa tcctgaagga tatggggatt
      121 acagaatatg agccaagagt tataaatcag atgttggagt ttgccttccg atatgtgacc
      181 acaattctag atgatgcaaa aatttattca agccatgcta agaaagctac tgttgatgca
      241 gatgatgtgc gattggcaat ccagtgccgc gctgatcagt cttttacctc tcctccccca
      301 agagattttt tattagatat tgcaaggcaa agaaatcaaa cccctttgcc attgatcaag
      361 ccatattcag gtcctaggtt gccacctgat agatactgct taacagctcc aaactatagg
      421 ctgaaatctt tacagaaaaa ggcatcaact tctgcgggaa gaataacagt cccgcggtta
      481 agtgttggtt cagttactag cagaccaagt actcccacac taggcacacc aaccccacag
      541 accatgtctg tttcaactaa agtagggact cccatgtccc tcacaggtca aaggtttaca
      601 gtacagatgc ctacttctca gtctccagct gtaaaagctt caattcctgc aacctcagca
      661 gttcagaatg ttctgattaa tccatcatta atcgggtcca aaaacattct tattaccact
      721 aatatgatgt catcacaaaa tactgccaat gaatcatcaa atgcattgaa aagaaaacgt
      781 gaagatgatg atgatgacga tgatgatgat gatgactatg ataatctgta cccaactttc
      841 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      901 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      961 tgtggaaagg acgaccacgt ctccgtatcg gagtgaat
//
