LOCUS       sequence-285672-        1739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3043
FEATURES             Location/Qualifiers
     source          1..1739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1571..1595)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1603..1644
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgatgag agagctgaac
       61 gcggattcat gctcaagccc ccagatgggg gctatgtggg agactagtgg gtctgtgaaa
      121 gagaactcca gtcagagtaa gaaatacagc acaaaaatag agaatcttgg tcctgagagc
      181 gcttgcaggc acttctggag cttccgttat catgaagcaa ccggaccgct tgagactatc
      241 agccaacttc agaaattgtg ccatcagtgg ctgaggccag agatccactc aaaagagcag
      301 atcttggaaa tgctggtgtt agagcagttc ctgagcattc tgcccaagga gacccagaac
      361 tgggtgcaga agcatcatcc acagaatgtc aaacaggctc tggtcctggt ggaattcttg
      421 cagagggagc ctgatggaac aaagaatgag gtcacagccc atgagctggg aaaggaggca
      481 gtgctcttgg gaggaacagc agtggcccca ggcttcaagt ggaagccagc agagccccaa
      541 ccaatgggtg tgttccagaa agaatattgg aatacatacc gggtactaca agaacagctg
      601 ggctggaaca ctcacaaaga aacccagcct gtatatgaaa gagctgtgca tgatcaacag
      661 atgttagccc tttctgagca gaaaagaatc aaacactgga agatggcatc taaactcatc
      721 ctgcctgagt ccctgagttt gttgacattt gaagatgtgg ctgtgtattt ttctgaggaa
      781 gagtggcaat tattgaatcc tcttgagaag actctctaca atgatgtaat gcaggatatc
      841 tatgagactg tcatctctct agggttaaag ctaaaaaatg acactggaaa tgatcatcct
      901 atatctgttt ctacatcaga aatacaaaca tcaggatgcg aagtatcaaa aaagaccaga
      961 atgaaaattg cccagaaaac aatgggcagg gaaaatcctg gtgatacaca cagtgtacag
     1021 aaatggcatc gagcttttcc aaggaagaaa agaaagaaac ctgcaacttg taaacaagag
     1081 cttccaaaac ttatggatct tcatgggaaa ggccccacag gggagaaacc ttttaagtgt
     1141 caggaatgtg ggaaaagctt cagagttagc tctgatctta ttaaacacca cagaattcac
     1201 actggagaga aaccctataa atgtcaacaa tgtgacagga ggtttagatg gagttcagat
     1261 cttaataagc acttcatgac ccatcaagga ataaaaccat atagatgctc atggtgtggg
     1321 aaaagcttta gtcataacac aaatctacac acacaccaaa gaattcacac aggagagaag
     1381 ccctttaaat gtgatgaatg tggaaaaaga ttcattcaga actcccacct tattaaacac
     1441 cagagaactc acacaggtga gcagccttat acatgtagct tatgcaagag aaactttagt
     1501 aggcgatcga gccttcttag acaccagaaa ctccacagaa gaagggaagc atgtctagtg
     1561 tctccaaact tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1621 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1681 atttcttggc tttatatatc ttgtggaaag gacgaggtga tactgcgaac gacaatggc
//
