LOCUS       sequence-285675-        1760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3044
FEATURES             Location/Qualifiers
     source          1..1760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1592..1616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1624..1665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcccttgg gtcagaattg
       61 ttcagggatg tggccatagt cttctcccaa gaagaatggc agtggttggc acctgctcag
      121 agggatttgt acagagacgt gatgttggag acctacagca acctggtctc actgggtctt
      181 gccgtttcca aacctgatgt gatctccttc ctagagcaag gcaaagagcc ctggatggtg
      241 gagagagtgg tgtctggagg cctgtgtcca gtattggagt ccagatatga taccaaggaa
      301 ttatttccaa agcagcatgt ttatgaagtc gaatcacccc aatgggagat aatggaaagc
      361 cttacaagtt atggtcttga gtgttcaagt ttccaagatg attgggaatg cagaaaccag
      421 tttgacagac aacagggaaa tccagacaga catttccatc agatgatcat cagacatgaa
      481 gaaatgccca cttttgacca gcatgcatcc cttacttttt atcagaaaat tcatactaga
      541 gaaaaacctt ttgggtataa taaatgtaga aaagacttct ggcaaaagga actccttatt
      601 aatcatcaag gaatttatac taatgagaaa ccctataaat gtaaggaatg tgggaaggcc
      661 tttaaatatg gctcacgact aattcaacat gagaatattc attctggtaa gaaaccatat
      721 gaatgtaagg aatgtggaaa ggccttcaat tctggttcaa attttataca acatcagaga
      781 gttcatactg gtgagaaacc gtatgaatgt aaagattgtg agaaggcctt tagtcgaagc
      841 tcacagttga ttgaacatca gcgaactcac acaggcgaga aaccctatca gtgtaaggaa
      901 tgtggcaagg ccttcaatcg gatctcacat cttaaagtac attatagaat tcatactggt
      961 gaaaaaccct atgcatgcaa ggaatgtggg aaaaccttta gtcatcgttc tcagttgatt
     1021 caacatcaga ctgttcatac tggcaggaaa ctctatgaat gtaaggaatg tgggaaggct
     1081 tttaatcaag gctcaactct tatacgacat cagagaattc ataccggtga gaaaccctat
     1141 gagtgcaaag tatgtggaaa ggcctttaga gtgagctcac aactcaagca acatcagaga
     1201 attcacactg gagagaaacc ctaccaatgt aaggtatgtg gtagggcctt caaacgggtc
     1261 tcacatctta ctgtacatta cagaattcat acaggtgaaa agccatatga atgtaaggaa
     1321 tgtgggaagg cttttagtca ttgctcacaa ttgattcatc atcaggttat tcacactgag
     1381 aaaaagccct atgaatataa ggaatgtgag aagaccttga gtcatgattc aactaccgtt
     1441 caacctcaga gaatgcataa tagagaaaca catgtgaata taataaatgt agaaaagcct
     1501 tccatcagca gttacccctt actaatcatc agagaattta tgttggcaag caaccatatg
     1561 aatggaagta atggggaaag cccattagcc ttgccaactt tcttgtacaa agtggttgat
     1621 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1681 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacat
     1741 accttcagta gccgcctccc
//
