LOCUS       sequence-285678-        1817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3045
FEATURES             Location/Qualifiers
     source          1..1817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1649..1673)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1681..1722
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgctcac cagcttttta
       61 cctgcaccta ctcagctatc tcaggaccag cttgaggctg aagaaaaggc aagatcccag
      121 agatcacggc agacctcact ggtctcctcc cgaagagaac ctcccccgta cggataccgg
      181 aaaggctgga tacctcggtt attagaggat tttggagatg gaggtgcttt tccagagatc
      241 catgtggccc agtatccact ggatatggga cgaaagaaaa aaatgtcgaa tgcgctggcc
      301 attcaggtgg attctgaagg aaaaattaaa tatgatgcaa ttgctcgaca aggacagtca
      361 aaagacaagg tcatttatag caaatacact gacctggttc caaaggaggt tatgaatgca
      421 gatgatccag acctgcaaag gcccgatgaa gaagctatta aagagataac agaaaagaca
      481 agagtagcct tagaaaaatc tgtatcacag aaggtcgccg cagccatgcc agttcgagca
      541 gctgacaaat tggctcctgc tcagtatatc cgatacacac catctcagca aggagtggca
      601 ttcaactctg gagctaaaca gagggttatt cggatggtag aaatgcagaa agatccaatg
      661 gagcctccaa ggttcaagat taataagaaa attccccggg gaccaccttc tcctcctgcg
      721 cctgtcatgc attctcctag ccgaaagatg actgtaaagg aacaacaaga gtggaagatt
      781 cctccttgta tttctaactg gaaaaatgca aagggttata caattccatt agacaaacgt
      841 ctggctgctg atggaagagg actacagaca gtacacataa atgaaaattt cgccaaattg
      901 gcagaagccc tctacattgc tgatcggaag gctcgtgaag ctgtggaaat gcgtgcccaa
      961 gtagagagaa aaatggctca gaaagaaaag gaaaaacatg aagagaaact tagagaaatg
     1021 gcccagaaag ccagggaaag aagagctggg atcaaaactc atgtggaaaa agaggatggg
     1081 gaggcacgtg agagggatga aatccggcat gacaggcgaa aagagagaca gcatgaccgg
     1141 aatctttcca gggcagctcc tgataagagg tcgaaacttc agagaaatga aaatcgggat
     1201 atcagtgaag ttattgctct cggtgttcct aatcctcgga cttccaatga agttcagtat
     1261 gaccaaaggc tcttcaacca atccaagggt atggacagtg gatttgcagg tggagaagat
     1321 gaaatttata atgtttatga tcaagcctgg agaggtggta aagatatggc ccagagtatt
     1381 tataggccca gtaaaaatct ggacaaggac atgtatggtg atgacctaga agccagaata
     1441 aagaccaaca gatttgttcc cgacaaggag ttttctggtt cagaccgtag acagagaggc
     1501 cgagaaggac cagttcagtt tgaggaagat ccttttggtt tggacaagtt tttggaagaa
     1561 gccaaacagc atggtggctc taaaagaccc tcagatagca gccgccccaa ggaacacgag
     1621 catgaaggca agaagaggag gaaggaattg ccaactttct tgtacaaagt ggttgatatc
     1681 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1741 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacgagggt
     1801 atcgtattac atgaact
//
