LOCUS       sequence-285681-        2237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3046
FEATURES             Location/Qualifiers
     source          1..2237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2069..2093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2101..2142
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcagggcac tgtggcattt
       61 gaagatgtgg ctgtgaactt ttcccaggag gagtggagtc tccttagtga ggttcagaga
      121 tgcctttacc atgacgtgat gctggagaac tgggtactta tatcctccct gggttgttgg
      181 tgtggatcag aagatgagga ggcaccttct aagaagagca tttctataca aagagtgtct
      241 caggtcagca ctcctggggc aggtgtgtct cccaagaagg cccactcttg tgaaatgtgt
      301 ggcgcgatct tgggagacat tttgcacttg gcagatcatc aggggacaca tcacaagcag
      361 aaactgcaca ggtgtgaggc atgggggaat aaattgtatg atagttcaaa ccgtccgcac
      421 cagaatcagt accttggaga gaaaccctat agaagcagtg ttgaggaagc attgtttgtg
      481 aagaggtgta agttccatgt gtcagaggag tcatctatct tcattcagag tggaaaggac
      541 tttttgccca gctcaggatt actgctgcag gaggccactc acactgggga gaagtcaaac
      601 agcaaacctg agtgtgagtc tccctttcag tggggagata ctcattacag ctgtggagaa
      661 tgcatgaaac attctagcac caaacacgta tttgttcaac agcagagact tccctctaga
      721 gaggaatgtt attgctggga atgtgggaaa tcctttagca aatatgatag cgtcagtaat
      781 catcagagag ttcacactgg gaaaagacct tatgaatgtg gagaatgtgg gaaatctttt
      841 agtcataagg gcagccttgt tcagcatcag cgagttcaca ctgggaaaag accttatgaa
      901 tgtggagaat gtgggaaatc ttttagtcat aagggcagcc ttgttcagca tcagcgagtt
      961 catactggag aaagacctta tgagtgtgga gaatgtggga aatcttttag tcaaaatggt
     1021 actctcatta aacatcaacg agttcacact ggagaaagac cttatgagtg tgaagaatgt
     1081 gggaaatgtt ttactcagaa gggcaatctc attcaacatc aacgaggtca cactagtgaa
     1141 agaccttatg agtgtgaaga atgtggaaaa tgttttagtc aaaagggcac cctaactgaa
     1201 catcatcgag ttcacactag agaacgacct tatgagtgtg gagaatgtgg gaaatctttt
     1261 agtcgaaagg gacaccttag gaaccatcag cgaggtcaca ctggagaaag accttacgag
     1321 tgtggagaat gtgggaaatc ttttagtcga aagggcaacc tcattcagca tcagcgaagc
     1381 cacactggag aaaggcctta tgagtgtaga gagtgtagga aattatttag gggcaagtcc
     1441 cacctcattg aacaccagag agttcacact ggagaaaggc catatgaatg taatgaatgt
     1501 gggaaatcat ttcaagacag ctctgggttt cgtgttcatc agagagttca cactggagaa
     1561 aaaccgtttg agtgtagtga atgtgggaag tcatttcctc aaagctgttc cctccttcga
     1621 catcggagag ttcatactgg agaaaggcct tatgaatgtg gagaatgtgg aaagtcattt
     1681 catcagagct cttccctcct tcgacatcag aaaactcaca ctgcagaaag accttatgag
     1741 tgcagagaat gtgggaaatt cttctccagt ctccttgaac acaggagagt tcacactgga
     1801 gaaaggcctt atgaatgcag ggaatgtgga aaaacattta ctcgaaggtc tgcgcatttt
     1861 aaacatcaga gacttcatac tcgaggaaag ccttacgagt gcagcgaatg tgggaaatcc
     1921 tttgctgaaa ccttcagtct tactgaacac aggagagtac acactggaga aaggccttat
     1981 gagtgcagtg aatgtggaaa atcatttcat cgaagctctt ctctccttcg acatcagaga
     2041 gttcacacag aaagaagtcc ttacaagttg ccaactttct tgtacaaagt ggttgatatc
     2101 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     2161 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatgtttcc
     2221 ttcatatttc cgtgttg
//
