LOCUS       sequence-285684-        1856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3047
FEATURES             Location/Qualifiers
     source          1..1856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1688..1712)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1720..1761
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgctggagaa ctacaagaat
       61 ttggccacag taggatatca gctcttcaaa cccagtctga tctcttggct ggaacaagaa
      121 gagtctagga cagtgcagag aggtgatttc caagcttcag aatggaaagt gcaacttaaa
      181 accaaagagt tagcccttca gcaggatgtt ttgggggagc caacctccag tgggattcaa
      241 atgataggaa gccacaacgg aggggaggtc agtgatgtta agcaatgtgg agatgtctcc
      301 agtgaacact catgccttaa gacacatgtg agaactcaaa atagtgagaa cacatttgag
      361 tgttatctgt atggagtaga cttccttact ctgcacaaga aaacctctac tggagagcaa
      421 cgttctgtat ttagtcagtg tggaaaagcc ttcagcctga acccagatgt tgtttgccag
      481 agaacgtgca caggagagaa agcttttgat tgcagtgact ctgggaaatc cttcattaat
      541 cattcacacc ttcagggaca tttaagaact cacaatggag aaagtctcca tgaatggaag
      601 gaatgtggga gaggctttat tcactccaca gaccttgctg tgcgtataca aactcacagg
      661 tcagaaaaac cctacaaatg taaggaatgt ggaaaaggat ttagatattc tgcatacctt
      721 aatattcaca tgggaaccca cactggagac aatccctatg agtgtaagga gtgtgggaaa
      781 gccttcacca ggtcttgtca acttactcag cacagaaaaa ctcacactgg agagaaacct
      841 tataaatgta aggattgtgg gagagccttc actgtttcct cttgcttaag tcaacatatg
      901 aaaatccatg tgggtgagaa gccttatgaa tgcaaggaat gtgggatagc cttcactaga
      961 tcttctcaac ttactgaaca tttaaaaact cacactgcaa aggatccctt tgaatgtaag
     1021 atatgtggaa aatcctttag aaattcctca tgcctcagtg atcactttcg aattcacact
     1081 ggaataaaac cctataaatg taaggattgt gggaaagcct tcactcagaa ctcagacctt
     1141 actaagcatg cacgaactca cagtggagag aggccctatg aatgtaagga atgtggaaag
     1201 gcctttgcca gatcctctcg ccttagtgaa catacaagaa ctcacactgg agagaagcct
     1261 tttgaatgtg tcaaatgtgg gaaagccttt gctatttctt caaatcttag tggacatttg
     1321 agaattcaca ctggagagaa gccctttgag tgcctggaat gtggtaaagc atttacgcat
     1381 tcctccagtc ttaataatca catgcggacc cacagcgcca aaaaaccatt cacgtgtatg
     1441 gaatgtggca aagcctttaa gtttcccacg tgtgttaacc ttcacatgcg gatccacact
     1501 ggagaaaaac cctacaaatg taaacagtgt gggaaatcct tcagttactc caattcgttt
     1561 cagttacatg aacgaactca cactggagag aaaccctatg aatgtaagga gtgcgggaaa
     1621 gccttcagtt cttccagttc ctttcgaaat catgaaagaa ggcatgcgga tgagagactg
     1681 tcagcattgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1741 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1801 tcttggcttt atatatcttg tggaaaggac gaattatcgg tccgtctaac cgaagc
//
