LOCUS       sequence-285690-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3049
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(929..953)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             961..1002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aagattatac caaaatagag
       61 aaaattggag aaggtaccta tggagttgtg tataagggta gacacaaaac tacaggtcaa
      121 gtggtagcca tgaaaaaaat cagactagaa agtgaagagg aaggggttcc tagtactgca
      181 attcgggaaa tttctctatt aaaggaactt cgtcatccaa atatagtcag tcttcaggat
      241 gtgcttatgc aggattccag gttatatctc atctttgagt ttctttccat ggatctgaag
      301 aaatacttgg attctatccc tcctggtcag tacatggatt cttcacttgt taagagttat
      361 ttataccaaa tcctacaggg gattgtgttt tgtcactcta gaagagttct tcacagagac
      421 ttaaaacctc aaaatctctt gattgatgac aaaggaacaa ttaaactggc tgattttggc
      481 cttgccagag cttttggaat acctatcaga gtatatacac atgaggtagt aacactctgg
      541 tacagatctc cagaagtatt gctggggtca gctcgttact caactccagt tgacatttgg
      601 agtataggca ccatatttgc tgaactagca actaagaaac cacttttcca tggggattca
      661 gaaattgatc aactcttcag gattttcaga gctttgggca ctcccaataa tgaagtgtgg
      721 ccagaagtgg aatctttaca ggactataag aatacatttc ccaaatggaa accaggaagc
      781 ctagcatccc atgtcaaaaa cttggatgaa aatggcttgg atttgctctc gaaaatgtta
      841 atctatgatc cagccaaacg aatttctggc aaaatggcac tgaatcatcc atattttaat
      901 gatttggaca atcagattaa gaagatgtac ccaactttct tgtacaaagt ggttgatatc
      961 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1021 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacaccccc
     1081 cggcgtttaa actccaa
//
