LOCUS       sequence-285696-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3051
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(938..962)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             970..1011
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatggaaga ttataccaaa
       61 atagagaaaa ttggagaagg tacctatgga gttgtgtata agggtagaca caaaactaca
      121 ggtcaagtgg tagccatgaa aaaaatcaga ctagaaagtg aagaggaagg ggttcctagt
      181 actgcaattc gggaaatttc tctattaaag gaacttcgtc atccaaatat agtcagtctt
      241 caggatgtgc ttatgcagga ttccaggtta tatctcatct ttgagtttct ttccatggat
      301 ctgaagaaat acttggattc tatccctcct ggtcagtaca tggattcttc acttgttaag
      361 agttatttat accaaatcct acaggggatt gtgttttgtc actctagaag agttcttcac
      421 agagacttaa aacctcaaaa tctcttgatt gatgacaaag gaacaattaa actggctgat
      481 tttggccttg ccagagcttt tggaatacct atcagagtat atacacatga ggtagtaaca
      541 ctctggtaca gatctccaga agtattgctg gggtcagctc gttactcaac tccagttgac
      601 atttggagta taggcaccat atttgctgaa ctagcaacta agaaaccact tttccatggg
      661 gattcagaaa ttgatcaact cttcaggatt ttcagagctt tgggcactcc caataatgaa
      721 gtgtggccag aagtggaatc tttacaggac tataagaata catttcccaa atggaaacca
      781 ggaagcctag catcccatgt caaaaacttg gatgaaaatg gcttggattt gctctcgaaa
      841 atgttaatct atgatccagc caaacgaatt tctggcaaaa tggcactgaa tcatccatat
      901 tttaatgatt tggacaatca gattaagaag atgtaggacc cagctttctt gtacaaagtg
      961 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1021 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1081 gaaatgatgt gtttgcacgt ttcagc
//
