LOCUS       sequence-285708-        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3055
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1424..1448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1456..1497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgactcccaa cagtatgaca
       61 gaaaatggcc ttacagcctg ggacaaaccg aagcactgtc cagaccgaga acacgactgg
      121 aagctagtag gaatgtctga agcctgccta cataggaaga gccattcaga gaggcgcagc
      181 acgttgaaaa atgaacagtc gtcgccacat ctcatccaga ccacttggac tagctcaata
      241 ttccatctgg accatgatga tgtgaacgac cagagtgtct caagtgccca gaccttccaa
      301 acggaggaga agaaatgtaa agggtacatc cccagttact tagacaagga cgagctctgt
      361 gtagtgtgtg gtgacaaagc caccgggtat cactaccgct gtatcacgtg tgaaggctgc
      421 aagggtttct ttagaagaac cattcagaaa aatctccatc catcctattc ctgtaaatat
      481 gaaggaaaat gtgtcataga caaagtcacg cgaaatcagt gccaggaatg tcgctttaag
      541 aaatgcatct atgttggcat ggcaacagat ttggtgctgg atgacagcaa gaggctggcc
      601 aagaggaagc tgatagagga gaaccgggag aaaagacggc gggaagagct gcagaagtcc
      661 atcgggcaca agccagagcc cacagacgag gaatgggagc tcatcaaaac tgtcaccgaa
      721 gcccatgtgg cgaccaacgc ccaaggcagc cactggaagc aaaaacggaa attcctgcca
      781 gaagacattg gacaagcacc aatagtcaat gccccagaag gtggaaaggt tgacttggaa
      841 gccttcagcc attttacaaa aatcatcaca ccagcaatta ccagagtggt ggattttgcc
      901 aaaaagttgc ctatgttttg tgagctgcca tgtgaagacc agatcatcct cctcaaaggc
      961 tgctgcatgg agatcatgtc ccttcgcgct gctgtgcgct atgacccaga aagtgagact
     1021 ttaaccttga atggggaaat ggcagtgaca cggggccagc tgaaaaatgg gggtcttggg
     1081 gtggtgtcag acgccatctt tgacctgggc atgtctctgt cttctttcaa cctggatgac
     1141 actgaagtag ccctccttca ggccgtcctg ctgatgtctt cagatcgccc ggggcttgcc
     1201 tgtgttgaga gaatagaaaa gtaccaagat agtttcctgc tggcctttga acactatatc
     1261 aattaccgaa aacaccacgt gacacacttt tggccaaaac tcctgatgaa ggtgacagat
     1321 ctgcggatga taggagcctg ccatgccagc cgcttcctgc acatgaaggt ggaatgcccc
     1381 acagaactct tccccccttt gttcttggaa gtgttcgagg atttgccaac tttcttgtac
     1441 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1501 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1561 aaggacgact gaccgctgtt cataggtgcc cc
//
