LOCUS       sequence-285714-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3057
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(650..674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             682..723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gagacaagaa gagccccacc
       61 aggccgaagc ggcagccgaa gccgtcctcg gatgagggtt actgggactg tagcgtctgc
      121 accttccgga acagcgccga ggccttcaag tgcatgatgt gcgatgtgcg gaagggcacc
      181 tccacccgat ccacattgtt tgaagttatt gtgagtgcct cgaggaccaa ggaaccgttg
      241 aaatttccaa tttcaggaag gaaacctcga cctgtctccc agttggttgc acagcaggtt
      301 actcagcagt ttgtgcctcc tacacagtca aagaaagaga aaaaagataa agtagaaaaa
      361 gaaaaaagtg aaaaggaaac aactagcaaa aagaatagcc ataagaaaac caggccaaga
      421 ttgaaaaatg tggatcggag tagtgctcag catttggaag ttactgttgg agatctgaca
      481 gtcattatta cagactttaa ggagaaaaca aagtcaccgc ctgcatctag tgctgcctct
      541 gcagatcaac acagtcaaag cggctctagc tctgataaca cagagagagg aatgtccagg
      601 tcatcttcac ccagaggaga agcctcatca ttgaatggag aatctcatta cccaactttc
      661 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      721 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      781 tgtggaaagg acgacggtat tactatgagt gcgcaacc
//
