LOCUS       sequence-285720-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3059
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(851..875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             883..924
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tccgagactt cggggaaccc
       61 ggcccgagct ccgggaacgg cggcgggtac ggcggccccg cgcagccccc ggccgcagcg
      121 caggcagccc agcagaagtt ccacctggtg ccaagcatca acaccatgag tggcagtcag
      181 gagctgcagt ggatggtaca gcctcatttc ctggggccca gcagttaccc caggcctctg
      241 acctaccctc agtacagccc cccacaaccc cggccaggag tcatccgggc cctggggccg
      301 cctccagggg tacgtcgaag gccttgtgaa cagatcagcc cggaggaaga ggagcgccgc
      361 cgagtaaggc gcgagcggaa caagctggct gcggccaagt gcaggaaccg gaggaaggaa
      421 ctgaccgact tcctgcaggc ggagactgac aaactggaag atgagaaatc tgggctgcag
      481 cgagagattg aggagctgca gaagcagaag gagcgcctag agctggtgct ggaagcccac
      541 cgacccatct gcaaaatccc ggaaggagcc aaggaggggg acacaggcag taccagtggc
      601 accagcagcc caccagcccc ctgccgccct gtaccttgta tctccctttc cccagggcct
      661 gtgcttgaac ctgaggcact gcacaccccc acactcatga ccacaccctc cctaactcct
      721 ttcaccccca gcctggtctt cacctacccc agcactcctg agccttgtgc ctcagctcat
      781 cgcaagagta gcagcagcag cggagaccca tcctctgacc cccttggctc tccaaccctc
      841 ctcgctttgt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
      901 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
      961 atttcttggc tttatatatc ttgtggaaag gacgacttgt gccgatttaa ccgctggct
//
