LOCUS       sequence-285723-        2414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3060
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2246..2270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2278..2319
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctctgagtga gaactcggtt
       61 tttgcctatg aatcttctgt gcatagcacc aatgttttac tcagccttaa tgaccagcgg
      121 aagaaagatg tgctgtgcga tgtcaccatc tttgtggagg gacagcggtt ccgcgctcac
      181 cggtccgtgc tggcggcatg cagcagttac ttccactcaa gaatcgtagg ccaggctgat
      241 ggagagctga acattactct tccagaagag gtgacagtta aaggatttga acctttaatt
      301 cagtttgcct acactgctaa actgatttta agtaaagaga atgtggatga agtgtgcaaa
      361 tgtgtggagt ttttaagtgt acataatatt gaggaatcct gctttcagtt tctgaaattt
      421 aagtttttgg actccactgc agaccagcaa gaatgcccaa gaaaaaaatg cttttcatca
      481 cactgtcaga aaacagacct taaactttca cttttggacc agagggatct agaaactgat
      541 gaagtggagg aatttctgga aaataaaaat gttcagactc ctcagtgtaa actccgcagg
      601 tatcaaggaa atgcaaaagc ctcacctcct ctacaagaca gtgccagtca gacatatgag
      661 tccatgtgct tagagaagga tgctgctctg gccttgcctt ctttatgccc caaatacaga
      721 aaattccaaa aagcatttgg aactgacaga gtccgtactg gggaatctag tgtcaaagac
      781 attcatgctt ctgttcagcc aaatgaaagg tctgaaaatg aatgcctggg aggagtcccg
      841 gagtgtagag atttgcaggt gatgttaaaa tgtgacgaaa gtaaattagc aatggaacct
      901 gaagaaacga agaaagatcc tgcttctcag tgcccaactg aaaaatcaga agtgactcct
      961 ttcccccaca attcttccat agaccctcat ggactttatt ctttgtctct tttacacaca
     1021 tatgaccaat atggtgactt gaattttgct ggtatgcaaa acacaacagt gttaacagaa
     1081 aagcctttgt caggtacaga cgtccaagaa aaaacatttg gtgaaagtca ggatttacct
     1141 ttgaaatccg acttgggcac cagggaagat agtagtgttg catctagtga taggagtagt
     1201 gtggagcgag aagtggcaga acacctagca aaaggcttct ggagtgacat ttgcagcacg
     1261 gacactcctt gccaaatgca gttatcacct gctgtggcca aagatggctc agaacagatc
     1321 tcacagaaac ggtctgagtg tccgtggtta ggtatcagga ttagtgagag cccagaacca
     1381 ggtcaaagga ctttcacaac attaagttct gtcaactgcc cttttataag tactctgagt
     1441 actgaaggct gttcaagcaa tttggaaatt ggaaacgatg attatgtttc agaaccccag
     1501 caagaacctt gcccatatgc ttgtgtcatt agcttgggag acgactctga gacggacacc
     1561 gaaggagaca gtgaatcctg ttcagccaga gaacaagaat gtgaggtaaa actgccattc
     1621 aatgcacaac ggataatttc actgtctcga aatgattttc agtccttgtt gaaaatgcac
     1681 aagcttactc cagaacagct ggattgtatc catgatattc gaagaagaag taaaaacaga
     1741 attgctgcac agcgctgtcg caagagaaaa cttgactgta tacagaatct tgaatcagaa
     1801 attgagaagc tgcaaagtga aaaggagagc ttgttgaagg aaagagatca cattttgtca
     1861 actctgggtg agacaaagca gaacctaact ggactttgcc agaaagtttg taaagaagca
     1921 gctctgagtc aagaacaaat acagatactc gccaagtact cagctgcaga ttgcccactt
     1981 tcatttttaa tttctgaaaa agataaaagt actcctgatg gtgaactggc gttaccatca
     2041 attttcagtt tatctgaccg gcctccagca gtgctgcctc cctgtgccag aggaaacagt
     2101 gagcctggct acgcgcgagg gcaggagtcc cagcagatgt ccacagccac ctctgagcaa
     2161 gctgggcctg cggaacagtg tcgtcagagt ggtgggatct cagatttctg tcagcagatg
     2221 actgataaat gtactactga tgagtaccca actttcttgt acaaagtggt tgatatcggt
     2281 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     2341 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tacacctata
     2401 taattactgt gagt
//
