LOCUS       sequence-285726-        1442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3061
FEATURES             Location/Qualifiers
     source          1..1442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1274..1298)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1306..1347
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggctgaagg aagccgaggt
       61 ggccctacgt gtagcggggt gggtggcagg caggacccag tctccggcag tggcggctgc
      121 aactttccag agtatgagct tcccgagcta aatacgcgcg ctttccatgt gggcgccttt
      181 ggggagctgt ggcggggccg tctgcgcggg gccggggact tgtcgctgag ggagccgccg
      241 gcatccgctc tgcctgggag ccaggcagct gactccgacc gggaggatgc cgcggtggcc
      301 agggatctgg actgcagcct ggaggcggcg gctgagctga gggcggtgtg cggccttgat
      361 aaactgaaat gccttgagga cggtgaggat ccagaagtca ttccggagaa tactgacctg
      421 gtgactttgg gggttagaaa aaggttcttg gaacatcggg aagaaaccat tacaatagat
      481 cgagcctgca gacaagaaac attcgtttat gagatggagt cacatgccat aggaaaaaag
      541 cctgaaaatt cagcagacat gattgaagaa ggggagctta tcctatctgt gaatatcttg
      601 taccctgtta tatttcataa gcacaaagaa cacaaaccat accaaacaat gctggtgttg
      661 ggcagtcaaa aactcacaca actgagggat tcaattcgat gtgtcagtga cctccagatt
      721 ggtggtgaat tcagcaacac tcctgaccaa gcccctgagc acatcagcaa agacctatac
      781 aaatcagcct tcttttattt tgaaggaaca ttttataatg ataaaagata cccagaatgc
      841 agagatttga gcagaactat cattgagtgg tcagagtccc atgatagagg ctatggaaag
      901 tttcagactg ctagaatgga agatttcacc ttcaatgact tgtgtattaa actgggtttt
      961 ccttacttat actgtcatca gggagactgt gaacatgtca ttgtcattac tgacataagg
     1021 cttgtgcatc atgatgactg cttggatagg acattgtatc ccctccttat caagaagcat
     1081 tggctatgga ccagaaaatg ttttgtttgt aaaatgtata cagccagatg ggtgacgaac
     1141 aatgacagtt ttgcaccaga ggacccatgc ttcttttgtg atgtttgctt ccgaatgctg
     1201 cactatgatt cagaaggcaa caaactgggg gaattccttg cttatcctta tgttgatcct
     1261 ggaaccttta atttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1321 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1381 tcgatttctt ggctttatat atcttgtgga aaggacgact atcgtcgtgc aattatatcc
     1441 ct
//
