LOCUS       sequence-285732-         548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3063
FEATURES             Location/Qualifiers
     source          1..548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(380..404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             412..453
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgcaagaatc tgggactgag
       61 acaaaaagta acggttcagc catccagaat gggtcgggcg gcagcaacca cttactagag
      121 tgcggcggtc ttcgggaggg gcggtccaac ggagagacgc cggccgtgga catcggggca
      181 gctgacctcg cccacgccca gcagcagcag caacagtggc atctcataaa ccatcagccc
      241 tctaggagtc ccagcagttg gcttaagaga ctaatttcaa gcccttggga gttggaagtc
      301 ctgcaggtcc ccttgtgggg agcagttgct gagacgaaga tgagtggacc tgtgtgtcag
      361 cctaaccctt ccccattttg cccaactttc ttgtacaaag tggttgatat cggtaagcct
      421 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      481 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcaaaa cggtccgagt
      541 ccctaccg
//
